The Ministry of Education published the 《Ten years development planning of informatization of education (2011-2020)》 in the 13th March 2012 and pointed out that the construction of educational information was an important symbol to evaluate the education level of universities. And it’s also an important support for the education to develop rapidly and sustainably. Large-scale equipment training in University has always mainly applied to the teachers, multimedia courseware, textbook and so on. Therefore, there are problems such as teaching is not clear and students can’t understand. In order to promote the literacy of the information technology of teachers and students and expand the application of f information teaching to meet the needs of education reform and development. National Biological Experimental Teaching Demonstration Center of Jilin University relied on the existing platform, strengthened the construction of network teaching constantly, and focused on the development of large-scale equipment training used by digital resources. This paper used multifunctional micro plate reader as an example to introduce the method and significance of network experiment construction. __________________________ Yan Shao, Guodong Yan, Yulin Zhou, Jiahui Lu, Yan Liu*, School of Life Sciences, Jilin University, Changchun, 130012, China