Sulfidic spring in the gypsum karst system of Monte Conca (Italy): chemistry and microbiological evidences

The explorations and the scientific investigations of sulfide-rich caves have advanced the knowledge on the development of karst systems. Multi-disciplinary teams have recognized the importance of hydrogen-sulfide on the geochemistry and microbiology of these systems. Principi (1931) first proposed sulfuric acid-driven speleogenesis and suggested that some caves were created by the interaction of sulfidic waters with limestone. Galdenzi & Maruoka (2003) reported that many limestone caves contain small gypsum deposits formed by evaporation of sulfate-rich water on cave fills or walls. Moreover, they described the morphologic effects of the oxidation of H2S for the Frasassi Cave, where the limestone walls, exposed to the H2S vapors, are highly corroded and partially or completely covered by gypsum crusts. Hose & Pisarowicz (1999) reported Citation:

[1]  B. Jørgensen,et al.  Growth Pattern and Yield of a Chemoautotrophic Beggiatoa sp. in Oxygen-Sulfide Microgradients , 1986, Applied and environmental microbiology.

[2]  L. Hose,et al.  Observations from active sulfuric karst systems: Is the present the key to understanding Guadalupe Mountain speleogenesis? , 2006, Caves and karst of southeastern New Mexico.

[3]  B. Jørgensen,et al.  Motility patterns of filamentous sulfur bacteria, Beggiatoa spp. , 2011, FEMS microbiology ecology.

[4]  D. Nelson,et al.  High Nitrate Concentrations in Vacuolate, Autotrophic Marine Beggiatoa spp , 1996, Applied and environmental microbiology.

[5]  M. Wagner,et al.  Filamentous “Epsilonproteobacteria” Dominate Microbial Mats from Sulfidic Cave Springs , 2003, Applied and Environmental Microbiology.

[6]  T. C. Kane,et al.  A Chemoautotrophically Based Cave Ecosystem , 1996, Science.

[7]  F. Millero,et al.  Oxidation of H2S in seawater as a function of temperature, pH, and ionic strength. , 1987, Environmental science & technology.

[8]  L. Rahm,et al.  A model study of the effects of sulfide-oxidizing bacteria (Beggiatoa spp.) on phosphorus retention processes in hypoxic sediments: Implications for phosphorus management in the Baltic Sea , 2011 .

[9]  N. Pace,et al.  Molecular phylogentic analysis of a bacterial community in Sulphur River, Parker Cave, Kentucky , 1998 .

[10]  A. Lepidi,et al.  Involvement of bacteria in the origin of a newly described speleothem in the gypsum cave of grave grubbo (Crotone, Italy) , 2012 .

[11]  Daniel S. Jones,et al.  Community genomic analysis of an extremely acidophilic sulfur-oxidizing biofilm , 2011, The ISME Journal.

[12]  T. Risby,et al.  Analysis of sulfuric acid aerosol by negative ion chemical ionization mass spectrometry , 1980 .

[13]  A. Kaushik,et al.  Precipitation of iron in microbial mats of the spring waters of Borra Caves, Vishakapatnam, India: some geomicrobiological aspects , 2008 .

[14]  P. Bennett,et al.  Microbial contributions to cave formation: New insights into sulfuric acid speleogenesis , 2004 .

[15]  Yue-hua Hu,et al.  Preliminary proteomic analysis of Thiobacillus ferrooxidans growing on elemental sulphur and Fe2+ separately. , 2005, Journal of biochemistry and molecular biology.

[16]  J. G. Kuenen,et al.  Sulfide-oxidizing bacteria in the burrowing echinoid, Echinocardium cordatum (Echinodermata) , 1993 .

[17]  H. Schulz-Vogt,et al.  Sulfur Respiration in a Marine Chemolithoautotrophic Beggiatoa Strain , 2012, Front. Microbio..

[18]  M. Dopson,et al.  Biodiversity, metabolism and applications of acidophilic sulfur-metabolizing microorganisms. , 2012, Environmental microbiology.

[19]  S. Galdenzi,et al.  GYPSUM DEPOSITS IN THE FRASASSI CAVES, CENTRAL ITALY , 2003 .

[20]  O. Lev,et al.  Electrospray Ionization Mass Spectrometric Analysis of Aqueous Polysulfide Solutions , 2004 .

[21]  M. Kuypers,et al.  Polysulfides as Intermediates in the Oxidation of Sulfide to Sulfate by Beggiatoa spp , 2013, Applied and Environmental Microbiology.

[22]  Joel D. Cline,et al.  SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN SULFIDE IN NATURAL WATERS1 , 1969 .

[23]  T. C. Kane,et al.  Ecological Assessment and Geological Significance of Microbial Communities from Cesspool Cave, Virginia , 2001 .

[24]  A. Boetius,et al.  Biological and chemical sulfide oxidation in a Beggiatoa inhabited marine sediment , 2007, The ISME Journal.

[25]  G. Lavik,et al.  Physiological Adaptation of a Nitrate-Storing Beggiatoa sp. to Diel Cycling in a Phototrophic Hypersaline Mat , 2007, Applied and Environmental Microbiology.

[26]  L. Nielsen,et al.  Impact of Bacterial NO3− Transport on Sediment Biogeochemistry , 2005, Applied and Environmental Microbiology.

[27]  M. Menichetti,et al.  Geology and Biology of the Frasassi Caves in Central Italy: An Ecological Multi-disciplinary Study of a Hypogenic Underground Ecosystem. , 2000 .

[28]  D. Northup,et al.  Microbiology and geochemistry in a hydrogen-sulphide-rich karst environment , 2000 .

[29]  R. Amann,et al.  Phylogeny and distribution of nitrate-storing Beggiatoa spp. in coastal marine sediments. , 2003, Environmental microbiology.

[30]  R. Duran,et al.  Characterization of purple sulfur bacteria from the South Andros Black Hole cave system: highlights taxonomic problems for ecological studies among the genera Allochromatium and Thiocapsa. , 2005, Environmental microbiology.

[31]  Daniel S. Jones,et al.  Geomicrobiology of biovermiculations from the Frasassi cave system, Italy , 2008 .

[32]  J. Macalady,et al.  Dominant Microbial Populations in Limestone-Corroding Stream Biofilms, Frasassi Cave System, Italy , 2006, Applied and Environmental Microbiology.

[33]  R. Beinart,et al.  Thermodynamics and Kinetics of Sulfide Oxidation by Oxygen: A Look at Inorganically Controlled Reactions and Biologically Mediated Processes in the Environment , 2011, Front. Microbio..

[34]  S. Scuri,et al.  SULFIDIC GROUND-WATER CHEMISTRY IN THE FRASASSI CAVES, ITALY , 2008 .

[35]  A. Kamp,et al.  Anaerobic Sulfide Oxidation with Nitrate by a Freshwater Beggiatoa Enrichment Culture , 2006, Applied and Environmental Microbiology.

[36]  Daniel S. Jones,et al.  Extremely acidic, pendulous cave wall biofilms from the Frasassi cave system, Italy. , 2007, Environmental microbiology.

[37]  A. Engel OBSERVATIONS ON THE BIODIVERSITY OF SULFIDIC KARST HABITATS , 2007 .

[38]  G. Luther,et al.  Low-oxygen and chemical kinetic constraints on the geochemical niche of neutrophilic iron(II) oxidizing microorganisms , 2008 .

[39]  A. Lehninger Principles of Biochemistry , 1984 .

[40]  H. Barton,et al.  GEOMICROBIOLOGY IN CAVE ENVIRONMENTS: PAST, CURRENT AND FUTURE PERSPECTIVES , 2007 .