Application of self‐assembly techniques in the design of biocompatible protein microarray surfaces

This review focuses on the application of novel technologies for generating biocompatible surfaces for high‐throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self‐assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pertaining to spot homogeneity, morphology, biocompatibility and reproducibility.

[1]  Charles T. Campbell,et al.  Protein contact printing for a surface plasmon resonance biosensor with on-chip referencing , 2001 .

[2]  George M. Whitesides,et al.  Patterning Self-Assembled Monolayers: Applications in Materials Science , 1994 .

[3]  M. Tarlov,et al.  Supramolecular architectures for the functionalization of solid surfaces. , 1997, Advances in biophysics.

[4]  A V Soldatov,et al.  Adjustment of transfer tools for the production of micro- and macroarrays. , 2001, BioTechniques.

[5]  J. Hoheisel,et al.  Versatile derivatisation of solid support media for covalent bonding on DNA-microchips. , 1999, Nucleic acids research.

[6]  P. Brown,et al.  Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions , 2001, Genome Biology.

[7]  H. Lehrach,et al.  Protein microarrays for gene expression and antibody screening. , 1999, Analytical biochemistry.

[8]  M. Boyce-Jacino,et al.  A SNPshot: pharmacogenetics and the future of drug therapy. , 2000, Trends in biotechnology.

[9]  S. Schreiber,et al.  Printing proteins as microarrays for high-throughput function determination. , 2000, Science.

[10]  K. Nelson,et al.  Surface plasmon resonance measurement of binding and dissociation of wild-type and mutant streptavidin on mixed biotin-containing alkylthiolate monolayers , 1999 .

[11]  E Neumann,et al.  Incorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopy. , 1998, Biosensors & bioelectronics.

[12]  Wolfgang Knoll,et al.  Thiopeptide-supported lipid layers on solid substrates , 1997 .

[13]  S. Loefas,et al.  Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. , 1991, Analytical biochemistry.

[14]  W. Knoll,et al.  Complement hybridization from solution to surface-attached probe-oligonucleotides observed by surface-plasmon-field-enhanced fluorescence spectroscopy , 2000 .

[15]  P. Brown,et al.  Yeast microarrays for genome wide parallel genetic and gene expression analysis. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[16]  S. P. Fodor,et al.  Using oligonucleotide probe arrays to access genetic diversity. , 1995, BioTechniques.

[17]  Charles T. Campbell,et al.  Binding and Dissociation Kinetics of Wild-Type and Mutant Streptavidins on Mixed Biotin-Containing Alkylthiolate Monolayers , 2000 .