Screening and identification of aerobic denitrifier with nitrite as substrate

Enrichment of aerobic denitrifier in activated sludge system was investigated, using intermittent aeration mode and nitrite as substrate in sequencing batch reactor (SBR). 70% of TN removal was observed when DO was 4–5mg/L, which indicated that aerobic denitrification occurred in activated sludge system. 30 strains were isolated from activated sludge, 10 strains of which had a high TN removal efficiency of more than 60%. Especially, 90.25% of TN removal was observed by strain Y23. The life cycle of Y23 was determined as 4 days and aerobic denitrification was mainly occurred at logarithmic phase (12–36h). After 4-day cultivation, TN removal efficiency and maximum removal rate was 90.25% and 3.47mg/ (L·h), respectively. In whole aerobic denitrification process, only 0.32mg/L NO3−-N was accumulated. The characteristics of colony were circular, white and opaque, viscous and the cell of Y23 was rod, gram negative under microscope. Moreover, Y23 was identified as Acinetobacter sp. by 16S rDNA sequences analysis.

[1]  E. W. V. van Niel,et al.  Simultaneous Nitrification and Denitrification in Aerobic Chemostat Cultures of Thiosphaera pantotropha , 1988, Applied and environmental microbiology.

[2]  P. Bonin,et al.  Effects of oxygen on each step of denitrification on Pseudomonas nautica , 1989 .

[3]  J. Rijn,et al.  Aerobic and anaerobic biofiltration in an aquaculture unit—Nitrite accumulation as a result of nitrification and denitrification , 1990 .

[4]  J. Cornier,et al.  Study of Factors Controlling Nitrite Build-Up in Biological Processes for Water Nitrification , 1992 .

[5]  Ju S. Huang,et al.  Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough , 1995 .

[6]  J. Keller,et al.  Simultaneous nitrification and denitrification in bench-scale sequencing batch reactors , 1996 .

[7]  Alka Gupta.  Thiosphaera pantotropha: a sulphur bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification , 1997 .

[8]  G. Allan,et al.  The effects of nitrite on the short-term growth of silver perch (Bidyanus bidyanus). , 1998 .

[9]  S. Tseng,et al.  A novel double‐membrane system for simultaneous nitrification and denitrification in a single tank , 1999, Letters in applied microbiology.

[10]  F. Rainey,et al.  Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant. , 1999, International journal of systematic bacteriology.

[11]  J. Keller,et al.  Study of factors affecting simultaneous nitrification and denitrification (SND) , 1999 .

[12]  Grosell,et al.  Uptake and effects of nitrite in the marine teleost fish Platichthys flesus. , 2000, Aquatic toxicology.

[13]  Hao Ying,et al.  Denitrification in aquaculture systems: an example of a fuzzy logic control problem , 2000 .

[14]  C. Martinez,et al.  Acute effects of nitrite on ion regulation in two neotropical fish species. , 2002, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[15]  B. Das,et al.  Nitrite toxicity in Cirrhinus mrigala (Ham.): acute toxicity and sub-lethal effect on selected haematological parameters , 2004 .

[16]  Zhou Dan-dan.  Selection and enrichment of aerobic denitrifier in activated sludge system , 2005 .

[17]  L. Shao,et al.  Isolation and characterization of a novel aerobic denitrifier from shrimp pond , 2006 .