Serial Analysis of Gene Expression (SAGE)

Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed. This short tag, because of its defined presence in the sequence, is typically sufficient to uniquely identify every transcript in the sample. A comprehensive profile of gene expression in any sample can thus be generated. Absolute, rather than relative, measurements of RNA abundance levels are made, which will be useful in the reliable comparison of data between laboratories.Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point Serial Analysis of Gene Expression (SAGE) involves the generation of short fragments of DNA, or tags, from a defined point