Detection of Organophosphorus Pesticides with Colorimetry and Computer Image Analysis

Organophosphorus pesticides (OPs) represent a very important class of pesticides that are widely used in agriculture because of their relatively high-performance and moderate environmental persistence, hence the sensitive and specific detection of OPs is highly significant. Based on the inhibitory effect of acetylcholinesterase (AChE) induced by inhibitors, including OPs and carbamates, a colorimetric analysis was used for detection of OPs with computer image analysis of color density in CMYK (cyan, magenta, yellow and black) color space and non-linear modeling. The results showed that there was a gradually weakened trend of yellow intensity with the increase of the concentration of dichlorvos. The quantitative analysis of dichlorvos was achieved by Artificial Neural Network (ANN) modeling, and the results showed that the established model had a good predictive ability between training sets and predictive sets. Real cabbage samples containing dichlorvos were detected by colorimetry and gas chromatography (GC), respectively. The results showed that there was no significant difference between colorimetry and GC (P > 0.05). The experiments of accuracy, precision and repeatability revealed good performance for detection of OPs. AChE can also be inhibited by carbamates, and therefore this method has potential applications in real samples for OPs and carbamates because of high selectivity and sensitivity.

[1]  Min Zhang,et al.  Quantum dot-DNA aptamer conjugates coupled with capillary electrophoresis: A universal strategy for ratiometric detection of organophosphorus pesticides. , 2016, Talanta.

[2]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[3]  Ganesh Kumar Mani,et al.  Electronic noses for food quality : a review , 2015 .

[4]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[5]  Design, synthesis and characterization of a hexapeptide bio-inspired by acetylcholinesterase and its interaction with pesticide dichlorvos. , 2014, The Analyst.

[6]  Giovanna Marrazza,et al.  Piezoelectric Biosensors for Organophosphate and Carbamate Pesticides: A Review , 2014, Biosensors.

[7]  D. Barr,et al.  HPLC-MS/MS Method for the Measurement of Insecticide Degradates in Baby Food , 2014, Journal of agricultural and food chemistry.

[8]  Kate Grudpan,et al.  Applications of everyday IT and communications devices in modern analytical chemistry: A review. , 2015, Talanta.

[9]  Nianqiang Wu,et al.  Plasmon-enhanced optical sensors: a review. , 2015, The Analyst.

[10]  V. Adam,et al.  Capillary electrophoresis of quantum dots: Minireview , 2014, Electrophoresis.

[11]  H. Kromhout,et al.  Suicide and exposure to organophosphate insecticides: cause or effect? , 2005, American journal of industrial medicine.

[12]  R. B. Pringle,et al.  Testing of novel brain-penetrating oxime reactivators of acetylcholinesterase inhibited by nerve agent surrogates. , 2013, Chemico-biological interactions.

[13]  G. Theodoridis,et al.  Solid phase microextraction applied to the analysis of organophosphorus insecticides in fruits. , 2006, Chemosphere.

[14]  Farid E Ahmed,et al.  Detection of genetically modified organisms in foods. , 2002, Trends in biotechnology.

[15]  Yongning Wu,et al.  Determination of 49 organophosphorus pesticide residues and their metabolites in fish, egg, and milk by dual gas chromatography-dual pulse flame photometric detection with gel permeation chromatography cleanup. , 2012, Journal of agricultural and food chemistry.

[16]  Tao Shen,et al.  Developing a novel sensitive visual screening card for rapid detection of pesticide residues in food , 2013 .

[17]  T. Satake,et al.  A micro IrOx potentiometric sensor for direct determination of organophosphate pesticides , 2015 .

[18]  Martin S. Fridson,et al.  Trends , 1948, Bankmagazin.

[19]  S. A. Mohajeri,et al.  Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples. , 2014, Food chemistry.

[20]  Miroslav Pohanka,et al.  Photography by Cameras Integrated in Smartphones as a Tool for Analytical Chemistry Represented by an Butyrylcholinesterase Activity Assay , 2015, Sensors.

[21]  Liang Feng,et al.  Recent Progresses in Optical Colorimetric/Fluorometric Sensor Array , 2013 .

[22]  R. Liebert.  Compost , 2018, Psycurity.

[23]  M. Stoytcheva,et al.  Electrochemical Biosensors for Direct Determination of Organophosphorus Pesticides: A Review , 2015 .

[24]  Soo-Hyun Park,et al.  Colorimetric Sensor Array for White Wine Tasting , 2015, Sensors.

[25]  V. Vasić,et al.  Send Orders of Reprints at Reprints@benthamscience.net Acetylcholinesterase Inhibitors: Pharmacology and Toxicology , 2022 .

[26]  Wang Limin,et al.  Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody , 2013 .

[27]  Mohammad Bagher Menhaj,et al.  Training feedforward networks with the Marquardt algorithm , 1994, IEEE Trans. Neural Networks.

[28]  H. Kameoka,et al.  Inhibition of Acetylcholinesterase Activity by Monoterpenoids with a p-Menthane Skeleton , 1997 .

[29]  Brett I. Pletschke,et al.  A novel colorimetric sensor strip for the detection of glyphosate in water , 2015 .

[30]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[31]  Hong Wang,et al.  Monitoring of organophosphorus pesticides in vegetables using monoclonal antibody-based direct competitive ELISA followed by HPLC-MS/MS , 2012 .

[32]  Min Wei,et al.  Development of acetylcholinesterase biosensor based on platinum–carbon aerogels composite for determination of organophosphorus pesticides , 2014 .

[33]  Hongtao Lei,et al.  Conformational changes of hapten-protein conjugates resulting in improved broad-specificity and sensitivity of an ELISA for organophosphorus pesticides. , 2011, The Analyst.

[34]  Hengwei Lin,et al.  Colorimetric sensor array for detection and identification of organophosphorus and carbamate pesticides. , 2015, Analytical chemistry.

[35]  M. Yost,et al.  A sensitive LC-MS/MS method for measurement of organophosphorus pesticides and their oxygen analogs in air sampling matrices , 2014, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[36]  A. Yu,et al.  Integrated microwave processing system for the extraction of organophosphorus pesticides in fresh vegetables. , 2015, Talanta.

[37]  P. Renard,et al.  Reactivators of acetylcholinesterase inhibited by organophosphorus nerve agents. , 2012, Accounts of chemical research.

[38]  J. Irudayaraj,et al.  Surface-enhanced Raman spectroscopy applied to food safety. , 2013, Annual review of food science and technology.