Effects of 50-hertz electromagnetic fields on proliferation and on chromosomal alterations in human peripheral lymphocytes untreated or pretreated with chemical mutagens.

Cultivation of human peripheral lymphocytes (HPL) in the presence of 50-Hz electromagnetic fields (EMFs) does not alter the spontaneous frequencies of sister-chromatid exchanges (SCE) and of chromosomal aberrations (CA), but leads to an enhancement of the cell cycle progression of HPLs in vitro. Pretreatment of HPLs with trenimon (TRN), diepoxybutane (DEB), or methylnitrosourea (MNU) in the G0 phase of the cell cycle results in dose-dependent elevations of the SCE frequencies. In some cases culturing of HPLs pretreated with MNU or TRN in the presence of EMFs led to significantly higher frequencies of SCEs when compared to cells cultivated in the absence of EMFs. Since we did not use multiple fixation times these data may rather result from differential influences on HPL subsets than from EMF exposure.

[1]  S. Nordström,et al.  Reproductive hazards among workers at high voltage substations. , 1983, Bioelectromagnetics.

[2]  N. Wertheimer,et al.  Adult cancer related to electrical wires near the home. , 1982, International journal of epidemiology.

[3]  M M Cohen,et al.  Effect of low-level, 60-Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes. , 1986, Bioelectromagnetics.

[4]  N. Wertheimer,et al.  Electrical wiring configurations and childhood cancer. , 1979, American journal of epidemiology.

[5]  D. Vågerö,et al.  Incidence of cancer in the electronics industry: using the new Swedish Cancer Environment Registry as a screening instrument. , 1983, British journal of industrial medicine.

[6]  R Hauf,et al.  Analysis of structural chromosome changes and SCE after occupational long-term exposure to electric and magnetic fields from 380 kV-systems , 1981, Radiation and environmental biophysics.

[7]  D. Scott,et al.  Changes in spontaneous SCE frequencies as a function of sampling time in lymphocytes from normal donors and cancer patients. , 1985, Mutation research.

[8]  J P Fulton,et al.  Electrical wiring configurations and childhood leukemia in Rhode Island. , 1980, American journal of epidemiology.

[9]  R. Smith,et al.  Effect of extremely-low-frequency pulsed magnetic fields on the mitogenic response of peripheral blood mononuclear cells. , 1986, Bioelectromagnetics.

[10]  M M Cohen,et al.  The effect of low-level 60-Hz electromagnetic fields on human lymphoid cells. II. Sister-chromatid exchanges in peripheral lymphocytes and lymphoblastoid cell lines. , 1986, Mutation research.

[11]  MichelP. Coleman,et al.  LEUKAEMIA INCIDENCE IN ELECTRICAL WORKERS , 1983, The Lancet.

[12]  S. Wolff,et al.  Increased induction of sister chromatid exchange by diethylstilbestrol in lymphocytes from pregnant and premenopausal women. , 1982, Cancer research.

[13]  W. Wright,et al.  LEUKAEMIA IN WORKERS EXPOSED TO ELECTRICAL AND MAGNETIC FIELDS , 1982, The Lancet.

[14]  S. Milham,et al.  Mortality from leukemia in workers exposed to electrical and magnetic fields. , 1982, The New England journal of medicine.

[15]  M. Reale,et al.  Reduced mitogenic stimulation of human lymphocytes by extremely low frequency electromagnetic fields , 1983, FEBS letters.

[16]  M. Mcdowall,et al.  LEUKAEMIA MORTALITY IN ELECTRICAL WORKERS IN ENGLAND AND WALES , 1983, The Lancet.