A rapid technique for detection of resistance to chloramphenicol in Streptococcus pneumoniae and comparison with minimum inhibitory concentration and disk-diffusion methods.

Fifty-two strains of Streptococcus pneumoniae were examined for production of chloramphenicol acetyltransferase (CAT) by a rapid technique based upon induction of enzyme activity and chemical assay. This method was compared with one measuring the minimum inhibitory concentration (MIC) by agar dilution and a diffusion test with disks containing 10 micrograms, 30 micrograms and 50 micrograms of chloramphenicol. The MIC for 13 chloramphenicol-resistant strains was 16 mg/L and for 39 sensitive strains less than or equal to 4 mg/L. The chemical assay clearly distinguished resistant from sensitive strains; it was technically simple and provided results within 90 min. The distinction between sensitive and resistant bacteria in the disk diffusion assay was clearer with 10-micrograms than with 30-microgram and 50-micrograms disks. However, the chemical CAT assay, with enzyme induction, is recommended when a rapid result is required.

[1]  D. Brown,et al.  The reliability of methods for detecting chloramphenicol resistance in Haemophilus influenzae. , 1988, Journal of Antimicrobial Chemotherapy.

[2]  C. Baker,et al.  Relationship between in vitro susceptibility test results for chloramphenicol and production of chloramphenicol acetyltransferase by Haemophilus influenzae, Streptococcus pneumoniae, and Aerococcus species , 1988, Journal of clinical microbiology.

[3]  S. Perry,et al.  Antimicrobial susceptibility testing of Streptococcus pneumoniae: quality assessment results. , 1988, Journal of clinical pathology.

[4]  R. George,et al.  Not the first multiresistant pneumococcus in Britain , 1987 .

[5]  F. K. Gould,et al.  A hospital outbreak of antibiotic-resistant Streptococcus pneumoniae. , 1987, The Journal of infection.

[6]  C. Latorre,et al.  Antibiotic resistance and serotypes of 100 Streptococcus pneumoniae strains isolated in a children's hospital in Barcelona, Spain , 1985, Antimicrobial Agents and Chemotherapy.

[7]  A. Wiesenthal,et al.  MULTIPLY RESISTANT PNEUMOCOCCUS CAUSING MENINGITIS: ITS EPIDEMIOLOGY WITHIN A DAY-CARE CENTRE , 1981, The Lancet.

[8]  J. Watts,et al.  STREPTOCOCCUS PNEUMONIAE RESISTANT TO PENICILLIN AND CHLORAMPHENICOL IN THE U.K. , 1981, The Lancet.

[9]  T. Stull,et al.  Rapid detection of chloramphenicol resistance in Haemophilus influenzae , 1981, Antimicrobial Agents and Chemotherapy.

[10]  W. V. Shaw,et al.  Chloramphenicol Resistance in Streptococcus pneumoniae: Enzymatic Acetylation and Possible Plasmid Linkage , 1978, Antimicrobial Agents and Chemotherapy.

[11]  W. V. Shaw.  Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. , 1975, Methods in enzymology.

[12]  J. Sherris,et al.  Antibiotic sensitivity testing. Report of an international collaborative study. , 1971, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[13]  J. Jeljaszewicz,et al.  Prevalence of types of Diplococcus pneumoniae and their susceptibility to 30 antibiotics. , 1970, Chemotherapy.