Hybridization of Polynucleotides Using Self-Assembled Monolayer Containing Pyrenyl Groups

To realize an efficient hybridization of polynucleotides, self-assembled monolayer (SAM) containing pyrene groups was prepared on a gold substrate because pyrene molecules have an affinity for nucleic acid bases through van der Waals interaction. The SAM was prepared from 11-(1-pyrene)-1-undeacathiol and 11-mercapto-1-undecanol mixed solution. The mixed SAM gave an efficient hybridization of polyadenylic acid and polyuridylic acid observed by surface plasmon resonance.