The purpose of this study was to describe the human larynx embryology, using three-dimensional reconstructions from seven human embryos histological sections, and to compare our results with literature. Seven human embryos from two historic French collections (Paris-Descartes University's Delmas-Rouviere collection and Rouen University's Tardif collection) stained with hematoxylin & eosin or Masson's trichrome were reclassified according to the Carnegie Atlas criteria (CS (Carnegie stages) 17, 18, 19, 21 and 22). A local made computer software developed by IADI lab (INSERM U947, Nancy) allowed automatic slices selection, automatic rigid registration, and dust filtering. Once registration had been completed, DICOM files were used with ORS Visual software (v1.5, Montreal), which allowed multiple display and post-processing possibilities (3D VR; orthogonal/curved multiplanar reformation). Due to large size files, image processing was performed on PC with Windows 7 (64 bit) and 20 GB RAM. Three-dimensiona...