Control of macromolecular synthesis in proliferating and resting syrian hamster cells in monolayer culture. II. Ribosome complement in resting and early G1 Cells

Using a new, sensitive and quantitative technique for determining the ribosomal‐RNA content of a measured number of cells, the cellular ribosome complement was compared for cultured hamster embryo cells in the stationary growth phase and in the early G1 phase of the cell cycle. Cells from stationary phase cultures were found to contain less than 70% of the ribosome complement of the early G1 phase cells, though the volumes of the two cell types were similar. This would imply that the stationary phase cell is physiologically different from a cell merely arrested at some point in the cell cycle.

[1]  C. Stanners,et al.  Control of macromolecular synthesis in proliferating and resting syrian hamster cells in monolayer culture. I. Ribosome function , 1971, Journal of cellular physiology.

[2]  M. D. Enger,et al.  RNA SYNTHESIS IN CHINESE HAMSTER CELLS , 1969, The Journal of cell biology.

[3]  Z. Darżynkiewicz,et al.  Autoradiographic studies on the stability of ribonucleic acid in phytohaemagglutinin stimulated lymphocytes. , 1969, Experimental cell research.

[4]  V. Terskikh,et al.  ON THE RESTING PERIODS IN THE CELL LIFE CYCLE * , 1969 .

[5]  A. Rubin.  Possible Control of Lymphocyte Growth at the Level of Ribosome Assembly , 1968, Nature.

[6]  D. Prescott.  Regulation of cell reproduction. , 1968, Cancer research.

[7]  R. Baserga.  BIOCHEMISTRY OF THE CELL CYCLE: A REVIEW , 1968 .

[8]  L. Tolmach,et al.  RNA synthesis in synchronously growing populations of HeLa S3 cells. I. Rate of total RNA synthesis and its relationship to DNA synthesis , 1968 .

[9]  H. M. Patt,et al.  CYTOKINETIC ANALYSIS OF TUMOR GROWTH* , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[10]  E. Robbins,et al.  Synthesis of Ribosomal RNA in Synchronized Hela Cells , 1965, Nature.

[11]  K. Nilausen,et al.  Reversible arrest of growth in G1 of an established fibroblast line (3T3). , 1965, Experimental cell research.

[12]  H. M. Patt,et al.  Radiation effects on cell renewal and related systems. , 1963, Physiological reviews.

[13]  L. Tolmach,et al.  Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. , 1963, Experimental cell research.

[14]  J. Till,et al.  DNA synthesis in individual L-strain mouse cells. , 1960, Biochimica et biophysica acta.

[15]  R. Dulbecco,et al.  PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES , 1954, The Journal of experimental medicine.

[16]  B. Leventhal,et al.  Effects of inhibition of ribosomal RNA synthesis on the stimulation of lymphocytes by phytohaemagglutinin. , 1969, Experimental cell research.

[17]  C. Epstein,et al.  A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry. , 1968, Analytical biochemistry.

[18]  U. Loening.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. , 1967, The Biochemical journal.