HPTLC Determination of Stigmasterol and Tocopherol Acetate in Leptadenia reticulata and in its Formulation

Leptadenia reticulata W and A (Jivanti) is a shrub of the Asclepiadaceae family with many medicinal uses. Two high-performance thin-layer chromatographic methods have been developed for separate quantitative analysis of stigmasterol and dl-α-tocopherol acetate, two marker compounds in Leptadenia reticulata. The methods are rapid, simple and accurate and can be used for routine quality testing.