Chitosan-Enriched Solution Blow Spun Poly(Ethylene Oxide) Nanofibers with Poly(Dimethylsiloxane) Hydrophobic Outer Layer for Skin Healing and Regeneration

Chitosan (CS)/poly(ethylene oxide) (PEO)-based nanofiber mats have attracted particular attention as advanced materials for medical and pharmaceutical applications. In the scope of present studies, solution blow spinning was applied to produce nanofibers from PEO and CS and physicochemical and biopharmaceutical studies were carried out to investigate their potential as wound nanomaterial for skin healing and regeneration. Additional coating with hydrophobic poly(dimethylsiloxane) was applied to favor removal of nanofibers from the wound surface. Unmodified nanofibers displayed highly porous structure with the presence of uniform, randomly aligned nanofibers, in contrast to coated materials in which almost all the free spaces were filled in with poly(dimethylsiloxane). Infrared spectroscopy indicated that solution blow technique did not influence the molecular nature of native polymers. Obtained nanofibers exhibited sufficient wound exudate absorbency, which appears beneficial to moisturize the wound bed during the healing process. Formulations displayed greater tensile strength as compared to commercial hydrofiber-like dressing materials comprised of carboxymethylcellulose sodium or calcium alginate, which points toward their protective function against mechanical stress. Coating with hydrophobic poly(dimethylsiloxane) (applied to favor nanofiber removal from the wound surface) impacted porosity and decreased both mechanical properties and adherence to excised human skin, though the obtained values were comparable to those attained for commercial hydrofiber-like materials. In vitro cytotoxicity and irritancy studies showed biocompatibility and no skin irritant response of nanofibers in contact with a reconstituted three-dimensional human skin model, while scratch assay using human fibroblast cell line HDFa revealed the valuable potential of CS/PEO nanofibers to promote cell migration at an early stage of injury.

[1]  A. Grumezescu,et al.  An Up-to-Date Review of Biomaterials Application in Wound Management , 2022, Polymers.

[2]  P. Makvandi,et al.  Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect , 2021, Bioengineering & translational medicine.

[3]  Jianan Song,et al.  Mass Production of Ultrafine Fibers by a Versatile Solution Blow Spinning Method , 2021 .

[4]  T. Rashid,et al.  Mechanical Properties of Electrospun Fibers—A Critical Review , 2021, Advanced Engineering Materials.

[5]  E. Drioli,et al.  Nano- and Micro-Porous Chitosan Membranes for Human Epidermal Stratification and Differentiation , 2021, Membranes.

[6]  P. Sajkiewicz,et al.  Solution Blow Spinning of Polycaprolactone—Rheological Determination of Spinnability and the Effect of Processing Conditions on Fiber Diameter and Alignment , 2021, Materials.

[7]  M. Krzyżowska,et al.  Potential of mucoadhesive chitosan glutamate microparticles as microbicide carriers – antiherpes activity and penetration behavior across the human vaginal epithelium , 2021, Drug delivery.

[8]  K. Winnicka,et al.  Tragacanth Gum/Chitosan Polyelectrolyte Complexes-Based Hydrogels Enriched with Xanthan Gum as Promising Materials for Buccal Application , 2020, Materials.

[9]  Bethwel K. Tarus,et al.  Investigation of mechanical properties of electrospun poly (vinyl chloride) polymer nanoengineered composite , 2020 .

[10]  Peifang Li,et al.  The Effect of Chitosan in Wound Healing: A Systematic Review. , 2020, Advances in skin & wound care.

[11]  J. A. Cecilia,et al.  Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications , 2020, Molecules.

[12]  T. Ciach,et al.  Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering , 2020, International journal of molecular sciences.

[13]  Xiao Hu,et al.  Effects of Fiber Density and Strain Rate on the Mechanical Properties of Electrospun Polycaprolactone Nanofiber Mats , 2020, Frontiers in Chemistry.

[14]  Yakai Feng,et al.  Polysaccharide Based Hemostatic Strategy for Ultrarapid Hemostasis. , 2020, Macromolecular bioscience.

[15]  Xiaowen Shi,et al.  Applications of chitin and chitosan nanofibers in bone regenerative engineering. , 2020, Carbohydrate polymers.

[16]  Heungsoo Shin,et al.  Current progress in application of polymeric nanofibers to tissue engineering , 2019, Nano Convergence.

[17]  M. Markuszewski,et al.  Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers? The effect of process variables on the in vitro functional and physicochemical characteristics. , 2019, International journal of pharmaceutics.

[18]  Y. Ni,et al.  Chitosan-based Polymer Matrix for Pharmaceutical Excipients and Drug Delivery. , 2019, Current medicinal chemistry.

[19]  A. Sannino,et al.  Influence of Nanofiber Orientation on Morphological and Mechanical Properties of Electrospun Chitosan Mats , 2018, Journal of healthcare engineering.

[20]  J. Costa,et al.  Development of electrospun nanofibers containing chitosan/PEO blend and phenolic compounds with antibacterial activity. , 2018, International journal of biological macromolecules.

[21]  Yan Liu,et al.  Tunable sequential drug delivery system based on chitosan/hyaluronic acid hydrogels and PLGA microspheres for management of non-healing infected wounds. , 2018, Materials science & engineering. C, Materials for biological applications.

[22]  Ju Lv,et al.  Nanofibrous polystyrene membranes prepared through solution blow spinning with an airbrush and the facile application in oil recovery , 2018 .

[23]  Michael K Danquah,et al.  Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations , 2018, Beilstein journal of nanotechnology.

[24]  M. Másson,et al.  Antimicrobial Chitosan and Chitosan Derivatives: A Review of the Structure-Activity Relationship. , 2017, Biomacromolecules.

[25]  Kirti Shitiz,et al.  Chitin and chitosan: biopolymers for wound management , 2017, International wound journal.

[26]  Y. Long,et al.  Electrospun nanofibers for wound healing. , 2017, Materials science & engineering. C, Materials for biological applications.

[27]  A. Chianese,et al.  Anti-Inflammatory, Immunomodulatory, and Tissue Repair Activity on Human Keratinocytes by Green Innovative Nanocomposites , 2017, Materials.

[28]  Adam M. Behrens,et al.  A Review of the Fundamental Principles and Applications of Solution Blow Spinning. , 2016, ACS applied materials & interfaces.

[29]  F. Bossard,et al.  Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide) , 2016, International journal of molecular sciences.

[30]  Z. Lavrič,et al.  Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies , 2016, Marine drugs.

[31]  J. Shaw,et al.  Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications. , 2016, International journal of pharmaceutics.

[32]  W. Tutak,et al.  Blow-spun chitosan/PEG/PLGA nanofibers as a novel tissue engineering scaffold with antibacterial properties , 2016, Journal of Materials Science: Materials in Medicine.

[33]  K. Winnicka,et al.  The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application , 2015 .

[34]  Xingyou Hu,et al.  Composite chitosan/poly(ethylene oxide) electrospun nanofibrous mats as novel wound dressing matrixes for the controlled release of drugs , 2015 .

[35]  B. Cheng,et al.  Solution blowing of chitosan/PVA hydrogel nanofiber mats. , 2014, Carbohydrate polymers.

[36]  Michael D. Buschmann,et al.  Chitosans for delivery of nucleic acids , 2013, Advanced Drug Delivery Reviews.

[37]  B. Ding,et al.  Biomimetic electrospun nanofibrous structures for tissue engineering. , 2013, Materials today.

[38]  K. Bekkour,et al.  The Effect of Temperature on the Rheological Behavior of Polyethylene Oxide (Peo) Solutions , 2013 .

[39]  I. Pucić,et al.  FTIR assessment of poly(ethylene oxide) irradiated in solid state, melt and aqeuous solution , 2012 .

[40]  Pharmaceutical Press.  Handbook of Pharmaceutical Excipients , 2012 .

[41]  H. Kandárová,et al.  In Vitro Skin Irritation Testing: Improving the Sensitivity of the EpiDerm Skin Irritation Test Protocol , 2009, Alternatives to laboratory animals : ATLA.

[42]  S. Percival,et al.  In vitro study of sustained antimicrobial activity of a new silver alginate dressing. , 2009, The journal of the American College of Certified Wound Specialists.

[43]  H. Kandárová,et al.  An in vitro skin irritation test (SIT) using the EpiDerm reconstructed human epidermal (RHE) model. , 2009, Journal of visualized experiments : JoVE.

[44]  Shing Chung Josh Wong,et al.  Effect of fiber diameter on tensile properties of electrospun poly(ɛ-caprolactone) , 2008 .

[45]  W. Krause,et al.  Correlation of chitosan's rheological properties and its ability to electrospin. , 2007, Biomacromolecules.

[46]  C. Liang,et al.  In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro , 2007, Nature Protocols.

[47]  F. Gottrup,et al.  Biophysical effects of repetitive removal of adhesive dressings on peri-ulcer skin. , 2006, Journal of wound care.

[48]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[49]  E J Wood,et al.  The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro. , 2001, Biomaterials.

[50]  P. Fram,et al.  The development of a novel technique for predicting the exudate handling properties of modern wound dressings. , 2001, Journal of tissue viability.

[51]  J. Desbrières,et al.  Influence of acetic acid concentration on the solubilization of chitosan , 1999 .

[52]  James B. Mitchell,et al.  Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. , 1987, Cancer research.

[53]  Xue Luo,et al.  Chitosan’s Wide Profile from Fibre to Fabrics: An Overview , 2019 .

[54]  T. Phillips,et al.  Choosing a Wound Dressing Based on Common Wound Characteristics. , 2016, Advances in wound care.

[55]  J. Rosiak,et al.  DETERMINATION OF DEGREE OF DEACETYLATION OF CHITOSAN - COMPARISION OF METHODS , 2012 .

[56]  R. White,et al.  Skin adhesives and their role in wound dressings , 2007 .

[57]  Kevin Woo,et al.  The role of moisture balance in wound healing. , 2007, Advances in skin & wound care.