Human salivary alpha-amylase reactivity in a psychosocial stress paradigm.

Biological indicators for stress reactions are valuable markers in psychophysiological research and clinical practice. Since the release of salivary enzyme alpha-amylase was reported to react to physiological and psychological stressors, we set out to investigate human salivary alpha-amylase changes employing a reliable laboratory stress protocol to investigate the reactivity of salivary alpha-amylase to a brief period of psychosocial stress. In a within-subject repeated-measures design, 24 healthy adults were exposed to the TSST and a control condition on separate days with randomized sequence. Salivary alpha-amylase, salivary cortisol and heart rate were repeatedly measured before, during and after both conditions. Significant differences between psychosocial stress and the rest condition in alpha-amylase activity [F(3.74,86.06)=4.52; P=0.003], cortisol levels [F(4.21,88.32)=12.48; P<0.001] and heart rate [F(1,22)=81.15; P<0.001] were observed, with marked increases before and after stress. The data corroborate findings from other studies that showed increased levels of alpha-amylase before and after psychological stress. We discuss the role of salivary alpha-amylase as a promising candidate for a reliable, noninvasive marker of psychosocial stress.

[1]  R. Biersner,et al.  Effects of Exercise Stress on Parotid Gland Secretion , 1979, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.

[2]  D. Morse,et al.  Salivary volume and amylase activity. I: Relaxation versus relaxed chewing. , 1983, The Journal of the American Society of Psychosomatic Dentistry and Medicine.

[3]  K O Schwab,et al.  Free epinephrine, norepinephrine and dopamine in saliva and plasma of healthy adults. , 1992, European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies.

[4]  S. Humphrey,et al.  A review of saliva: normal composition, flow, and function. , 2001, The Journal of prosthetic dentistry.

[5]  D. Morse,et al.  Stress, relaxation, and saliva: a pilot study involving endodontic patients. , 1981, Oral surgery, oral medicine, and oral pathology.

[6]  R. Pangborn,et al.  Mastication and its influence on human salivary flow and alpha-amylase secretion , 1990, Physiology & Behavior.

[7]  C. Kirschbaum,et al.  Two formulas for computation of the area under the curve represent measures of total hormone concentration versus time-dependent change , 2003, Psychoneuroendocrinology.

[8]  G. Medema,et al.  Salivary levels of immunoglobulin A in triathletes. , 1997, European journal of oral sciences.

[9]  C. Kirschbaum,et al.  The 'Trier Social Stress Test'--a tool for investigating psychobiological stress responses in a laboratory setting. , 1993, Neuropsychobiology.

[10]  C. Kirschbaum,et al.  Salivary cortisol in psychoneuroendocrine research: Recent developments and applications , 1994, Psychoneuroendocrinology.

[11]  M. Levine,et al.  Salivary α-Amylase: Role in Dental Plaque and Caries Formation , 1993 .

[12]  G A Hudgens,et al.  Hormonal responses to psychological stress in men preparing for skydiving. , 1997, The Journal of clinical endocrinology and metabolism.

[13]  J. Bugajski,et al.  Adrenergic regulation of the hypothalamic-pituitary-adrenal axis under basal and social stress conditions. , 1995, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[14]  C. Kirschbaum,et al.  Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement , 1992, The Journal of Steroid Biochemistry and Molecular Biology.

[15]  Gerald A. Hudgens,et al.  Salivary α‐amylase as a measure of endogenous adrenergic activity , 1996 .

[16]  E. Shirtcliff,et al.  Use of salivary biomarkers in biobehavioral research: cotton-based sample collection methods can interfere with salivary immunoassay results , 2001, Psychoneuroendocrinology.

[17]  T. Kamarck,et al.  A global measure of perceived stress. , 1983, Journal of health and social behavior.

[18]  D. Morse,et al.  Stress, meditation and saliva: a study of separate salivary gland secretions in endodontic patients. , 1983, Journal of oral medicine.

[19]  B. Baum,et al.  Principles of Saliva Secretion , 1993, Annals of the New York Academy of Sciences.

[20]  Speirs Rl.  SALIVA AND DENTAL HEALTH , 1984 .

[21]  Cees G. M. Snoek,et al.  Protein composition of whole and parotid saliva in healthy and periodontitis subjects. Determination of cystatins, albumin, amylase and IgA. , 1996, Journal of periodontal research.

[22]  J. Herman,et al.  Excitatory Influence of the Locus Coeruleus in Hypothalamic‐Pituitary‐Adrenocortical Axis Responses to Stress , 1999, Journal of neuroendocrinology.

[23]  J. R. Garrett.  Effects of Autonomic Nerve Stimulations on Salivary Parenchyma and Protein Secretion , 1999 .

[24]  D. Morse,et al.  Stress, relaxation and saliva: a follow-up study involving clinical endodontic patients. , 1981, Journal of human stress.

[25]  J. Goldberg,et al.  The effect of stress and meditation on salivary protein and bacteria: a review and pilot study. , 1982, Journal of human stress.

[26]  P. Mills,et al.  Catecholamines in human saliva. , 2001, Life sciences.

[27]  P. Csermely,et al.  Hsp70 is present in human saliva. , 2003, Medical science monitor : international medical journal of experimental and clinical research.

[28]  J. R. Garrett,et al.  Structural and functional studies of the effects of parasympathetic nerve stimulation on rabbit submandibular salivary glands. , 1986, Archives of oral biology.

[29]  B. Bermond,et al.  Psychological Stress as a Determinant of Protein Levels and Salivary-Induced Aggregation of Streptococcus gordonii in Human Whole Saliva , 1996, Psychosomatic medicine.

[30]  C. Dawes,et al.  Saliva and Oral Health , 2004 .

[31]  D. Bruns,et al.  Biochemistry of human alpha amylase isoenzymes. , 1985, Critical reviews in clinical laboratory sciences.

[32]  C. Schneyer,et al.  Effects of Varying Frequency of Sympathetic Stimulation on Chloride and Amylase Levels of Saliva Elicited from Rat Parotid Gland with Electrical Stimulation of both Autonomic Nerves , 1991, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[33]  B. Bermond,et al.  The response of salivary protein levels and S-IgA to an academic examination are associated with daily stress , 1998 .

[34]  E. Nexo,et al.  Human salivary epidermal growth factor, haptocorrin and amylase before and after prolonged exercise. , 1988, Scandinavian journal of clinical and laboratory investigation.

[35]  F. Borgeat,et al.  Comparison of the salivary changes associated with a relaxing and with a stressful procedure. , 1984, Psychophysiology.

[36]  Sohee Park,et al.  Modulation of attentional inhibition by norepinephrine and cortisol after psychological stress. , 2000, International journal of psychophysiology : official journal of the International Organization of Psychophysiology.

[37]  Arie V Nieuw Amerongen,et al.  Innate Secretory Immunity in Response to Laboratory Stressors That Evoke Distinct Patterns of Cardiac Autonomic Activity , 2003, Psychosomatic medicine.

[38]  B. Kudielka,et al.  Impact of gender, menstrual cycle phase, and oral contraceptives on the activity of the hypothalamus-pituitary-adrenal axis. , 1999, Psychosomatic medicine.

[39]  Edgar Erdfelder,et al.  GPOWER: a priori, post-hoc, and compromise power analyses for MS-DOS [Computer Program] , 2004 .

[40]  R. Biersner,et al.  Human Parotid Gland Alpha-Amylase Secretion as a Function of Chronic Hyperbaric Exposure , 1979 .

[41]  W. Edgar.  Saliva and dental health. Clinical implications of saliva: report of a consensus meeting , 1990, British Dental Journal.

[42]  P. Robson,et al.  The effects of high-intensity intermittent exercise on saliva IgA, total protein and alpha-amylase. , 1999, Journal of sports sciences.