ACIDIC, OSMOTIC AND ULTRASONIC RESISTANCE OF ERYTHROCYTES IN REGULAR HEMODIALYSIS PATIENTS

THE AIM of the investigation was to assess the reaction of erythrocytes to uremia and hemodialysis treatment based on many years of studying different types of hemolysis. PATIENTS AND METHODS. The examination included 108 patients with chronic renal failure (66 men and 42 women; mean age 43.29±1.22 years). The patients received 200 sessions of standard bicarbonate dislysis. The control group consisted of 27 healthy subjects (17 men and 12 women; mean age 46.2±3.1 years). The time of acidic, osmotic and ultrasonic hemolysis was studied as well as the osmotic resistance of erythrocytes. RESULTS. The time of acidic hemolysis in patients before the beginning of the hemodialysis session was 33.8% longer as compared with the same index for the control group (t=12.4; p<0.001). Although the time of acidic hemolysis was 11.8% shorter due to the hemodialysis session, it was still 18% higher (t=7.3; p<0.001). The osmotic resistance in patients before dialysis was 6.5% higher than normal (t=2.35; p<0.05), and after dialysis it was 9.23% reliably higher (t=5.04; p<0.001). The duration of osmotic and ultrasonic hemolysis before and after dialysis did not reliably differ from the similar indices of the control group. Weak negative correlation was revealed between the time of acidic hemolysis and content of calcium and albumin in the patients’ blood plasma after hemodialysis session. The correlation coefficients were (cid:2)0.206± 0.070 (t=2.96; p<0.01) and (cid:2)0.216±0.069 (t=3.11; p<0/001) respectively. The first factor with the greatest weight included the indices of arterial pressure before and after the hemodialysis session showing the state of the patients’ cardiovascular system. The second component with the relative weight a little more than 8% characterized the effectiveness of the hemodialysis session. The third factor included the concentration of albumine and cholesterol in blood plasma. The fourth factor consisted of the indices associated exclusively with the acidic and ultrasonic resistance of erythrocytes.The indices of osmotic resistance were included but in the ninth factor. CONCLUSION. An analysis of the data on the erythrocyte resistance to acidic and osmotic hemolysis showing the functional state of red blood cells as a whole and, in particular, their membranes, has revealed a reliably positive influence of the hemodialysis procedure on the cellular sector of organism of the dialysis treated patients. It was shown that in the course of the hemodialysis session the acidic hemolysis time shortened and the osmotic resistance of erythrocytes considerably increased. The correlation and factor analysis of the data obtained has confirmed high degree of autonomy of erythrocytes. For all this, the indices of different types of hemolysis, being somewhat inferior by their informative value to clinical and biochemical indices, nevertheless have sufficiently high factor significance.