Coherent anti-Stokes Raman scattering (RP-CARS) microscopy for sensing molecular orientations

We report coherent anti-Stokes Raman scattering (CARS) microscopy with radially polarized (RP) excitation for facilitating longitudinally oriented molecules detection. Our finite-difference time-domain (FDTD) simulation results show that the maximum near-field RP-CARS radiation from the two longitudinally orientated nanoparticles is around 1.3 times higher than that from transversely orientated nanoparticles. Our further CARS experiments show that RP-CARS radiation from molecules oriented along the longitudinal direction is approximately 3-fold stronger than that using linearly polarized CARS technique, and the lateral resolution of RP-CARS imaging can be improved by about 10% compared to the linearly polarized CARS imaging.

[1]  Wei Zheng,et al.  Interferometric polarization coherent anti-Stokes Raman scattering (IP-CARS) microscopy. , 2008, Optics letters.

[2]  Eric O. Potma,et al.  Detection of single lipid bilayers with coherent anti‐Stokes Raman scattering (CARS) microscopy , 2003 .

[3]  Q. Zhan.  Cylindrical vector beams: from mathematical concepts to applications , 2009 .

[4]  G Leuchs,et al.  Sharper focus for a radially polarized light beam. , 2003, Physical review letters.

[5]  C. Sheppard,et al.  Numerical study of effects of light polarization, scatterer sizes and orientations on near-field coherent anti-Stokes Raman scattering microscopy. , 2009, Optics express.

[6]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[7]  Tsutomu Araki,et al.  Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation. , 2007, Optics letters.

[8]  H. A. Rinia,et al.  Measuring molecular order and orientation using coherent anti-stokes Raman scattering microscopy. , 2007, Methods in molecular biology.

[9]  X. Xie,et al.  Polarization coherent anti-Stokes Raman scattering microscopy. , 2001, Optics letters.

[10]  Ji-Xin Cheng,et al.  Molecular composition and orientation in myelin figures characterized by coherent anti-stokes Raman scattering microscopy. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[11]  Andreas Volkmer,et al.  Vibrational imaging and microspectroscopies based on coherent anti-Stokes Raman scattering microscopy , 2005 .

[12]  T G Brown,et al.  Longitudinal field modes probed by single molecules. , 2001, Physical review letters.

[13]  Wei Zheng,et al.  Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light. , 2009, Optics letters.

[14]  Andreas Zumbusch,et al.  Coherent anti-Stokes Raman scattering microscopy , 1999 .

[15]  Jane A Dickerson,et al.  Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine. , 2008, Annual review of analytical chemistry.

[16]  K. Yee.  Numerical solution of initial boundary value problems involving maxwell's equations in isotropic media , 1966 .

[17]  S.,et al.  Numerical Solution of Initial Boundary Value Problems Involving Maxwell’s Equations in Isotropic Media , 1966 .