The Preliminary Characterization of the β-Glucosidases of Cellulomonas fimi

β-Glucosidase activity in Cellulomonas fimi is strictly intracellular. Cell-free extracts contain at least two β-glucosidases. One of the enzymes is constitutive and it hydrolyses p -nitrophenyl-β-D-glucoside (PNPG) but not cellobiose. A second enzyme is induced about fourfold by growth on Avicel, and about sevenfold by growth on cellobiose; it hydrolyses both PNPG and cellobiose.

[1]  D. Kilburn,et al.  Isolation and characterization of Escherichia coli clones expressing cellulase genes from Cellulomonas fimi , 1984 .

[2]  D. Kilburn,et al.  The Cellulase System of Cellulomonas fimi , 1984 .

[3]  T. Wood,et al.  Purification and Some Properties of the Extracellular β-d-Glucosidase of the Cellulolytic Fungus Trichoderma koningii , 1982 .

[4]  L. Vining,et al.  Glucose suppression of beta-glucosidase activity in a chloramphenicol-producing strain of Streptomyces venezuelae. , 1982, Canadian journal of microbiology.

[5]  P. Rapp,et al.  Formation, Location, and Regulation of Endo-1,4-beta-Glucanases and beta-Glucosidases from Cellulomonas uda. , 1982, Applied and environmental microbiology.

[6]  R. Armentrout,et al.  Molecular Cloning of Genes for Cellobiose Utilization and Their Expression in Escherichia coli , 1981, Applied and environmental microbiology.

[7]  H. Meyer,et al.  Separation and Some Properties of Two Intracellular β-Glucosidases of Sporotrichum (Chrysosporium) thermophile , 1981, Applied and environmental microbiology.

[8]  N. Creuzet,et al.  Characterization and purification of thermostable beta-glucosidase from Clostridium thermocellum. , 1979, Biochemical and biophysical research communications.

[9]  G. T. Tsao,et al.  Cellulase and Biosynthesis Regulation , 1979 .

[10]  Hiroshi Suzuki,et al.  Intracellular Distribution and Some Properties of β-Glucosidases of a Cellulolytic Pseudomonad , 1976 .

[11]  T. Harada,et al.  Purification and properties of a β-1,6-glucosidase from Flavobacterium , 1975 .

[12]  V. R. Srinivasan,et al.  Purification and Characterization of β-Glucosidase of Alcaligenes faecalis , 1969, Journal of bacteriology.

[13]  K. Eriksson,et al.  Extracellular enzyme system utilized by the rot fungus Stereum sanguinolentum for the breakdown of cellulose. IV. Separation of cellobiase and aryl beta-glucosidase activities. , 1969, Archives of biochemistry and biophysics.

[14]  J. K. Alexander.  Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum. , 1968, The Journal of biological chemistry.

[15]  H. Takahashi,et al.  Fermentation of C14-Labeled Cellobiose by Cellulomonas fimi , 1967 .

[16]  M. Jermyn.  Fungal Cellulases IV. PRODUCTION AND PURIFICATION OF AN EXTRACELLULAR ,a-GLUCOSIDASE OFSTACHYBOTRYS ATRA , 1955 .

[17]  M. Jermyn.  Fungal Cellulases VI. SUBSTRATE AND INHIBITOR SPECIFICITY OF THE fl-GLUCOSIDASE OFST ACHYBOTRYS ATRA , 1955 .