Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR and analyzed. The genome size of Pigeon-CIAV-1906 was 2,298 bp with the highest homology (99.5%) to Jilin strain (JL14023) and the lowest homology (91.5%) to Brazil strain (KY024579), which phylogenetically clustered into Group A. Notably, several amino acids such as 139K and 394Q related with high virulence were found in the VP1 of Pigeon-CIAV-1906. The isolation of Pigeon-CIAV-1906 and its molecular characteristics provide evidence for the cross-transmission of CIAV from chicken to pigeon and give novel insights into the molecular epidemiology of CIAV.

[1]  Yang Li,et al.  Genomic Characterization of Recent Chicken Anemia Virus Isolates in China , 2017, Front. Microbiol..

[2]  R. L. Harrison,et al.  Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016) , 2016, Archives of Virology.

[3]  F. Chen,et al.  Identification of a Chicken Anemia Virus Variant-Related Gyrovirus in Stray Cats in China, 2012 , 2014, BioMed research international.

[4]  F. Chen,et al.  Complete Genome Sequence Analysis of a Recent Chicken Anemia Virus Isolate and Comparison with a Chicken Anemia Virus Isolate from Human Fecal Samples in China , 2012, Journal of Virology.

[5]  A. Omar,et al.  Detection and characterization of chicken anemia virus from commercial broiler breeder chickens , 2008, Virology Journal.

[6]  R. Lurz,et al.  Morphological characterization of chicken anaemia agent (CAA) , 2005, Archives of Virology.

[7]  A. Omar,et al.  Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells , 2003, Archives of Virology.

[8]  D. Jackson,et al.  Chicken Anemia Virus VP2 Is a Novel Dual Specificity Protein Phosphatase* , 2002, The Journal of Biological Chemistry.

[9]  B. Adair,et al.  Molecular Basis of the Attenuation Exhibited by Molecularly Cloned Highly Passaged Chicken Anemia Virus Isolates , 2002, Journal of Virology.

[10]  N. Tanimura,et al.  Identification of a genetic determinant of pathogenicity in chicken anaemia virus. , 2001, The Journal of general virology.

[11]  M. Noteborn,et al.  Simultaneous expression of recombinant baculovirus-encoded chicken anaemia virus (CAV) proteins VP1 and VP2 is required for formation of the CAV-specific neutralizing epitope. , 1998, The Journal of general virology.

[12]  J. Rosenberger,et al.  Chicken anemia virus. , 1998, Poultry science.

[13]  T. Hayashi,et al.  A serological survey of chickens, Japanese quail, pigeons, ducks and crows for antibodies to chicken anaemia virus (CAV) in Japan. , 1998, Avian pathology : journal of the W.V.P.A.

[14]  A. van der Eb,et al.  A single chicken anemia virus protein induces apoptosis , 1994, Journal of virology.

[15]  M. Noteborn,et al.  Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein. , 1992, Gene.

[16]  G. F. de Boer,et al.  Transient depletion of cortical thymocytes induced by chicken anaemia agent. , 1989, Thymus.

[17]  T. Taniguchi,et al.  Isolation and some characteristics of an agent inducing anemia in chicks. , 1979 .