Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)

We present a dual-wavelength fast mechanical scanning optical-resolution photoacoustic microscopy system (OR-PAM) in this paper. Conventional mechanical scanning OR-PAM system takes ~ 30 min for one frame of single wavelength imaging. The acquisition of two wavelengths will double the total imaging time. By using a fast-linear stage, a high signal-to-noise (SNR) optical and acoustic combiner and 2 lasers at different wavelengths, we achieved a B-scan rate of 12 Hz and reduced the acquisition time to ~1.5 min for dual-wavelength imaging. This system can be used in large field optical resolution imaging. Contrast-free vascular and functional imaging can be achieved using this system. Both ex vivo and in vivo imaging results are demonstrated.

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