Enhanced enzymatic hydrolysis of sugarcane bagasse by N-methylmorpholine-N-oxide pretreatment.

The cellulose dissolution solvent used in Lyocell process for cellulose fiber preparation, N-methylmorpholine-N-oxide (NMMO) monohydrate, was demonstrated to be an effective agent for sugarcane bagasse pretreatment. Bagasse of 20wt% was readily dissolved in NMMO monohydrate at 130 degrees C within 1h. After dissolution, bagasse could be regenerated by rapid precipitation with water as a porous and amorphous mixture of its original components. The regenerated bagasse exhibited a significant enhancement on enzymatic hydrolysis kinetic. Not only the reducing sugars releasing rate but also hydrolysis yield was enhanced at least twofold as compared with that of untreated bagasse. The cellulose fraction of regenerated bagasse was nearly hydrolyzed to glucose after 72h hydrolysis with Cellulase AP3. The recycled NMMO demonstrated the same performance as the fresh one on bagasse pretreatment for hydrolysis enhancement. The regenerated bagasse was directly used in simultaneous saccharification and fermentation (SSF) for ethanol production by Zymomonas mobilis. No negative effect on ethanol fermentation was observed and ethanol yield approximately 0.15 g ethanol/g baggasse was achieved.

[1]  R. Elander,et al.  Process and economic analysis of pretreatment technologies. , 2005, Bioresource technology.

[2]  H. Sixta,et al.  Cellulose solutions in N-methylmorpholine-N-oxide (NMMO) – degradation processes and stabilizers , 2002 .

[3]  T. K. Ghose.  Measurement of cellulase activities , 1987 .

[4]  M. L. Nelson,et al.  Relation of certain infrared bands to cellulose crystallinity and crystal lattice type. Part II. A new infrared ratio for estimation of crystallinity in celluloses I and II , 1964 .

[5]  Ye Sun,et al.  Hydrolysis of lignocellulosic materials for ethanol production: a review. , 2002, Bioresource technology.

[6]  J. M. Paturau,et al.  By-Products of the Cane Sugar Industry: An Introduction to their Industrial Utilization , 1969 .

[7]  G. Catignani,et al.  Antioxidant Properties of Lignin , 1982 .

[8]  Erich Adler,et al.  Lignin chemistry—past, present and future , 1977, Wood Science and Technology.

[9]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[10]  Lee R Lynd,et al.  A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure. , 2006, Biomacromolecules.

[11]  Chen Hongzhang,et al.  Enzymatic hydrolysis of cellulose materials treated with ionic liquid [BMIM] Cl , 2006 .

[12]  A. Ragauskas,et al.  Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature , 2008, Biotechnology and bioengineering.

[13]  J. Saddler,et al.  Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions, and antioxidant activity. , 2006, Journal of agricultural and food chemistry.

[14]  W. Park,et al.  Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy. , 2005, Carbohydrate research.

[15]  H. Fink,et al.  Structure formation of regenerated cellulose materials from NMMO-solutions , 2001 .

[16]  Vijay Kumar,et al.  Phosphoric acid mediated depolymerization and decrystallization of cellulose: Preparation of low crystallinity cellulose — A new pharmaceutical excipient , 1996 .

[17]  B. Kos̆iková,et al.  Stabilization effect of lignin in polypropylene and recycled polypropylene , 2005 .

[18]  C. Wyman,et al.  Features of promising technologies for pretreatment of lignocellulosic biomass. , 2005, Bioresource technology.

[19]  Johnathan E. Holladay,et al.  Interactions between cellulose and N-Methylmorpholine-N-oxide , 2007 .

[20]  Mark Laser,et al.  Fractionating recalcitrant lignocellulose at modest reaction conditions. , 2007, Biotechnology and bioengineering.

[21]  C. A. Schall,et al.  Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step , 2006, Biotechnology and bioengineering.