Cell sheets in cell therapies.

This review aims to provide a broad introduction to the use of cell sheets and the role of materials in the delivery of cell sheets to patients within a clinical setting. Traditionally, cells sheets have been, and currently are, fabricated using established and accepted cell culture methods within standard formats (e.g., petri dishes) utilizing biological substrates. Synthetic surfaces provide a far more versatile system for culturing and delivering cell sheets. This has the potential to positively affect quality, and efficient, localized cell delivery has a significant impact on patient outcome and on the overall cost of goods. We highlight current applications of these advanced carriers and future applications of these surfaces and cell sheets with an emphasis both on clinical use and regulatory requirements.

[1]  I. Kerkis,et al.  A novel strategy of mesenchymal stem cells delivery in the uterus of mares with endometrosis. , 2013, Theriogenology.

[2]  A. Vanderkelen,et al.  Feeder layer- and animal product-free culture of neonatal foreskin keratinocytes: improved performance, usability, quality and safety , 2011, Cell and Tissue Banking.

[3]  W. Chun,et al.  The application of cultured epithelial autografts improves survival in burns , 2015, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[4]  A. D. Celiz,et al.  Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture , 2014, Biomaterials science.

[5]  Robert Langer,et al.  Discovery of Novel Materials with Broad Resistance to Bacterial Attachment Using Combinatorial Polymer Microarrays , 2013, Advanced materials.

[6]  T. Okano,et al.  Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. , 2004, The New England journal of medicine.

[7]  Francesca Ungaro,et al.  Controlled drug delivery in tissue engineering. , 2008, Advanced drug delivery reviews.

[8]  S. MacNeil,et al.  Application of layer-by-layer coatings to tissue scaffolds - development of an angiogenic biomaterial. , 2014, Journal of materials chemistry. B.

[9]  T. Okano,et al.  Structural characterization of bioengineered human corneal endothelial cell sheets fabricated on temperature-responsive culture dishes. , 2006, Biomaterials.

[10]  Masayuki Yamato,et al.  Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. , 2005, Tissue engineering.

[11]  Nicola J Brown,et al.  Development of a reconstructed human skin model for angiogenesis , 2003, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[12]  Hiroyuki Honda,et al.  Construction and harvest of multilayered keratinocyte sheets using magnetite nanoparticles and magnetic force. , 2004, Tissue engineering.

[13]  Sheila MacNeil,et al.  Development of biodegradable electrospun scaffolds for dermal replacement. , 2008, Biomaterials.

[14]  Masayuki Yamato,et al.  Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface , 2004, Transplantation.

[15]  D. C. Adams,et al.  Grafts in dermatologic surgery: review and update on full- and split-thickness skin grafts, free cartilage grafts, and composite grafts. , 2006, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[16]  Louise E. Smith,et al.  Transplantation of oral mucosa explants using different biological and synthetic membranes in an attempt to achieve one-stage reconstruction of soft-tissue defects , 2012 .

[17]  G. Pellegrini,et al.  Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. , 2001, Transplantation.

[18]  U. Jonas,et al.  Rejuvenation in the early 20th century , 2009, Andrologia.

[19]  S. MacNeil,et al.  A Cell Therapy for Chronic Wounds Based Upon a Plasma Polymer Delivery Surface , 2006 .

[20]  S. MacNeil,et al.  Development of a surface-modified contact lens for the transfer of cultured limbal epithelial cells to the cornea for ocular surface diseases. , 2009, Tissue engineering. Part A.

[21]  R. Tsai,et al.  Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. , 2000, The New England journal of medicine.

[22]  W. Jeffcoate,et al.  Randomized, controlled, single-blind study on use of autologous keratinocytes on a transfer dressing to treat nonhealing diabetic ulcers. , 2007, Regenerative medicine.

[23]  Giles T S Kirby,et al.  Development of Advanced Dressings for the Delivery of Progenitor Cells. , 2017, ACS applied materials & interfaces.

[24]  S. Mac Neil,et al.  Development of autologous human dermal–epidermal composites based on sterilized human allodermis for clinical use , 1999, The British journal of dermatology.

[25]  Giles T S Kirby,et al.  Accelerating protein release from microparticles for regenerative medicine applications , 2013, Materials science & engineering. C, Materials for biological applications.

[26]  L. Ovington,et al.  Advances in wound dressings. , 2007, Clinics in dermatology.

[27]  Laura Suggs,et al.  A PEGylated fibrin patch for mesenchymal stem cell delivery. , 2006, Tissue engineering.

[28]  F. A. Navarro,et al.  Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model. , 2000, The Journal of burn care & rehabilitation.

[29]  H Green,et al.  Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. , 1975, Cell.

[30]  J. Rheinwald.  Chapter 15 Serial Cultivation of Normal Human Epidermal Keratinocytes , 1980 .

[31]  H. Green,et al.  Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell is , 1975, Cell.

[32]  A. Dulamea,et al.  Mesenchymal stem cells in multiple sclerosis - translation to clinical trials , 2015, Journal of medicine and life.

[33]  Masayuki Yamato,et al.  Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture. , 2002, Biomaterials.

[34]  J. Davies,et al.  Optimizing the sterilization of PLGA scaffolds for use in tissue engineering. , 2001, Biomaterials.

[35]  J. Fabré,et al.  The healing of chronic venous leg ulcers with prepared human amnion. , 1989, British journal of plastic surgery.

[36]  P. Hartman,et al.  Adaptation of plastic surfaces for tissue culture by glow discharge , 1975, Journal of clinical microbiology.

[37]  F. Wood,et al.  The use of cultured epithelial autograft in the treatment of major burn injuries: a critical review of the literature. , 2006, Burns : journal of the International Society for Burn Injuries.

[38]  T. Inatomi,et al.  Sterilized, freeze-dried amniotic membrane: a useful substrate for ocular surface reconstruction. , 2004, Investigative ophthalmology & visual science.

[39]  R E Horch,et al.  Single-cell suspensions of cultured human keratinocytes in fibrin-glue reconstitute the epidermis. , 1998, Cell transplantation.

[40]  I. Schwab,et al.  Inherent risks associated with manufacture of bioengineered ocular surface tissue. , 2006, Archives of ophthalmology.

[41]  S. MacNeil,et al.  Use of peracetic acid to sterilize human donor skin for production of acellular dermal matrices for clinical use , 2004, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[42]  Richard T. Lee,et al.  Biomaterials to enhance stem cell function in the heart. , 2011, Circulation research.

[43]  De-Quan Li,et al.  Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. , 2004, Experimental eye research.

[44]  Louise E. Smith,et al.  A chemically defined carrier for the delivery of human mesenchymal stem/stromal cells to skin wounds. , 2012, Tissue engineering. Part C, Methods.

[45]  Masayuki Yamato,et al.  Cell sheet engineering: recreating tissues without biodegradable scaffolds. , 2005, Biomaterials.

[46]  S. Tseng,et al.  Human Allograft Limbal Transplantation for Corneal Surface Reconstruction , 1994, Cornea.

[47]  T. Zwingers,et al.  Treatment of recalcitrant venous leg ulcers with autologous keratinocytes in fibrin sealant: A multinational randomized controlled clinical trial , 2007, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[48]  J. Ge,et al.  Cell delivery with fixed amniotic membrane reconstructs corneal epithelium in rabbits with limbal stem cell deficiency. , 2011, Investigative ophthalmology & visual science.

[49]  F. Wood,et al.  An alternative technique for the harvesting of cultured epithelial cell sheets , 1995 .

[50]  B. Atiyeh,et al.  Cultured epithelial autograft (CEA) in burn treatment: three decades later. , 2007, Burns : journal of the International Society for Burn Injuries.

[51]  Marcus Textor,et al.  Polyelectrolyte Coatings with a Potential for Electronic Control and Cell Sheet Engineering , 2008 .

[52]  Sheila MacNeil,et al.  Clinical experience using cultured epithelial autografts leads to an alternative methodology for transferring skin cells from the laboratory to the patient. , 2006, Regenerative medicine.

[53]  R. Short,et al.  Attachment of human keratinocytes to plasma co-polymers of acrylic acid/octa-1,7-diene and allyl amine/octa-1,7-diene , 1998 .

[54]  H. Bannasch,et al.  Treatment of therapy-refractive ulcera cruris of various origins with autologous keratinocytes in fibrin sealant. , 2005, VASA. Zeitschrift fur Gefasskrankheiten.

[55]  N. Koizumi,et al.  An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture. , 2002, Investigative ophthalmology & visual science.

[56]  S. E. James,et al.  A review of tissue-engineered skin bioconstructs available for skin reconstruction , 2010, Journal of The Royal Society Interface.

[57]  A. Michelmore,et al.  Role of positive ions in determining the deposition rate and film chemistry of continuous wave hexamethyl disiloxane plasmas. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[58]  E. Marin,et al.  Critical evaluation of biodegradable polymers used in nanodrugs , 2013, International journal of nanomedicine.

[59]  T. Okano,et al.  Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. , 2001, Tissue engineering.

[60]  T. Kamarul,et al.  Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells , 2011, Cell and Tissue Banking.

[61]  R. Boyd,et al.  Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy – a review , 2016, BMC Musculoskeletal Disorders.

[62]  P. Skládal,et al.  Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions , 2016 .

[63]  D. Siniscalco,et al.  Mesenchymal stem cell therapy for the treatment of chronic obstructive pulmonary disease , 2010, Expert opinion on biological therapy.

[64]  R. Short,et al.  Plasma Copolymerization of Allyl Alcohol/1,7-Octadiene: Surface Characterization and Attachment of Human Keratinocytes , 1998 .

[65]  Robert D. Short,et al.  Plasma Treatment of Polymers: The Effects of Energy Transfer from an Argon Plasma on the Surface Chemistry of Polystyrene, and Polypropylene. A High-Energy Resolution X-ray Photoelectron Spectroscopy Study , 1998 .

[66]  B. Min,et al.  Human amniotic membrane as a delivery matrix for articular cartilage repair. , 2007, Tissue engineering.

[67]  B. R. Coad,et al.  Hyperthermal Intact Molecular Ions Play Key Role in Retention of ATRP Surface Initiation Capability of Plasma Polymer Films from Ethyl α-Bromoisobutyrate. , 2016, ACS applied materials & interfaces.

[68]  H. Dua.  Amniotic membrane transplantation , 1999, The British journal of ophthalmology.

[69]  C. Compton,et al.  Permanent Coverage of Large Burn Wounds with Autologous Cultured Human Epithelium , 1984 .

[70]  Tsuyoshi Murata,et al.  {m , 1934, ACML.

[71]  Ching‐Shwun Lin.  Stem cell therapy for the bladder--where do we stand? , 2011, The Journal of urology.

[72]  Sheila MacNeil,et al.  Progress and opportunities for tissue-engineered skin , 2007, Nature.

[73]  G. Naughton,et al.  A metabolically active human dermal replacement for the treatment of diabetic foot ulcers. , 2008, Artificial organs.

[74]  T. Okano,et al.  Functional human corneal endothelial cell sheets harvested from temperature‐responsive culture surfaces , 2006, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[75]  K. Vasilev,et al.  A Mechanistic Study of the Plasma Polymerization of Ethanol , 2014 .

[76]  S. MacNeil,et al.  Developing biodegradable scaffolds for tissue engineering of the urethra , 2011, BJU international.

[77]  Mitsuo Umezu,et al.  Fabrication of Pulsatile Cardiac Tissue Grafts Using a Novel 3-Dimensional Cell Sheet Manipulation Technique and Temperature-Responsive Cell Culture Surfaces , 2002, Circulation research.

[78]  S. MacNeil,et al.  Tissue-engineered buccal mucosa urethroplasty-clinical outcomes. , 2008, European urology.

[79]  M. Subrahmanyam.  Amniotic membrane as a cover for microskin grafts. , 1995, British journal of plastic surgery.

[80]  R. Vohra,et al.  Fibronectin coating of expanded polytetrafluoroethylene (ePTFE) grafts and its role in endothelial seeding. , 2008, Artificial organs.

[81]  Robert Langer,et al.  Materials for stem cell factories of the future. , 2014, Nature materials.

[82]  T. Starzl.  History of Clinical Transplantation , 2000, World Journal of Surgery.

[83]  Louise E. Smith,et al.  Production of tissue-engineered skin and oral mucosa for clinical and experimental use. , 2011, Methods in molecular biology.

[84]  R. Short,et al.  Plasma copolymerization as a route to the fabrication of new surfaces with controlled amounts of specific chemical functionality , 1996 .

[85]  K. Remes.  Cell therapy , 2002, Journal of the Neurological Sciences.

[86]  M. Scheflan,et al.  Clinical trials of amniotic membranes in burn wound care. , 1982, Plastic and reconstructive surgery.

[87]  Fabrizio Gelain,et al.  Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections , 2008, BMC biotechnology.

[88]  M. Lee,et al.  Effects of human amniotic membrane grafts combined with marrow mesenchymal stem cells on healing of full-thickness skin defects in rabbits , 2009, Cell and Tissue Research.

[89]  Clifford Pereira,et al.  Review Paper: Burn Coverage Technologies: Current Concepts and Future Directions , 2007, Journal of biomaterials applications.

[90]  T. Okano,et al.  Transportation of transplantable cell sheets fabricated with temperature‐responsive culture surfaces for regenerative medicine , 2008, Journal of tissue engineering and regenerative medicine.

[91]  M. Vallet‐Regí,et al.  Vascular endothelial and smooth muscle cell culture on NaOH-treated poly(epsilon-caprolactone) films: a preliminary study for vascular graft development. , 2005, Macromolecular bioscience.

[92]  T. Okano,et al.  A noninvasive transfer system for polarized renal tubule epithelial cell sheets using temperature-responsive culture dishes. , 2005, European cells & materials.

[93]  R. Soetikno,et al.  Treatment of oesophageal ulcerations using endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets in a canine model , 2006, Gut.

[94]  Masayuki Yamato,et al.  Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues , 2006, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[95]  B. R. Coad,et al.  Chlorine-rich plasma polymer coating for the prevention of attachment of pathogenic fungal cells onto materials surfaces , 2016 .

[96]  J. Fisher,et al.  Biocompatibility and potential of acellular human amniotic membrane to support the attachment and proliferation of allogeneic cells. , 2008, Tissue engineering. Part A.

[97]  M. Textor,et al.  One-step method for generating PEG-like plasma polymer gradients: chemical characterization and analysis of protein interactions. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[98]  R. Short,et al.  Plasma polymerisation for molecular engineering of carbon-fibre surfaces for optimised composites , 1997 .

[99]  J R Wolfe,et al.  A New Method of Performing Plastic Operations , 1875, British medical journal.

[101]  A. Morey.  Tissue-engineered buccal mucosa for substitution urethroplasty. , 2005, The Journal of urology.

[102]  M. Griffith,et al.  Functional human corneal equivalents constructed from cell lines. , 1999, Science.

[103]  A. G. Bettman.  Homogenous thiersch grafting as a life saving measure , 1938 .

[104]  S. MacNeil,et al.  A new autologous keratinocyte dressing treatment for non‐healing diabetic neuropathic foot ulcers , 2004, Diabetic medicine : a journal of the British Diabetic Association.

[105]  R. MacLaren,et al.  Stem cell therapy and the retina , 2007, Eye.

[106]  D. Ratner.  SKIN GRAFTING: From Here to There , 1998 .

[107]  D. Hutmacher,et al.  Reduced contraction of skin equivalent engineered using cell sheets cultured in 3D matrices. , 2006, Biomaterials.

[108]  P. Quesenberry,et al.  Participation of bone marrow derived cells in cutaneous wound healing , 2003, Journal of cellular physiology.

[109]  S. MacNeil,et al.  The effect of adipose tissue derived MSCs delivered by a chemically defined carrier on full-thickness cutaneous wound healing. , 2013, Biomaterials.

[110]  F. Wood,et al.  Scar management of cultured epithelial autograft. , 1996, Burns : journal of the International Society for Burn Injuries.

[111]  A. Ratcliffe,et al.  Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films. , 1999, Biomaterials.

[112]  T. Okano,et al.  Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. , 1999, Journal of biomedical materials research.

[113]  S. MacNeil,et al.  Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair. , 2013, Acta biomaterialia.

[114]  T. Okano,et al.  Urothelium regeneration using viable cultured urothelial cell sheets grafted on demucosalized gastric flaps. , 2004, BJU international.

[115]  R. Lower.  An account of the experiment of transfusion, practiced upon a man in London. 1667. , 2002, The Yale Journal of Biology and Medicine.

[116]  Masayuki Yamato,et al.  Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs. , 2005, Journal of periodontal research.

[117]  Masayuki Yamato,et al.  Tissue engineered epithelial cell sheets for the creation of a bioartificial trachea. , 2006, Tissue engineering.

[118]  P. Cochat,et al.  Et al , 2008, Archives de pediatrie : organe officiel de la Societe francaise de pediatrie.

[119]  Dietmar W. Hutmacher,et al.  Biodegradable polymers applied in tissue engineering research: a review , 2007 .

[120]  T. Okano,et al.  Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface. , 2000, Biomacromolecules.

[121]  Masayuki Yamato,et al.  Cell sheet engineering for regenerative medicine: Current challenges and strategies , 2014, Biotechnology journal.

[122]  Masayuki Yamato,et al.  Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface. , 2005, Investigative ophthalmology & visual science.

[123]  F. Larcher,et al.  Large surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels. , 1998, Burns : journal of the International Society for Burn Injuries.

[124]  M. del Río,et al.  [Skin bioengineering: preclinical and clinical applications]. , 2012, Actas dermo-sifiliograficas.

[125]  S. Boyce,et al.  The 1999 clinical research award. Cultured skin substitutes combined with Integra Artificial Skin to replace native skin autograft and allograft for the closure of excised full-thickness burns. , 1999, The Journal of burn care & rehabilitation.

[126]  Janos Vörös,et al.  pH-controlled recovery of placenta-derived mesenchymal stem cell sheets. , 2011, Biomaterials.

[127]  D. Hutmacher,et al.  In vitro characterization of natural and synthetic dermal matrices cultured with human dermal fibroblasts. , 2004, Biomaterials.

[128]  M. M. Ghosh,et al.  A Comparison of Methodologies for the Preparation of Human Epidermal‐Dermal Composites , 1997, Annals of plastic surgery.

[129]  T. Okano,et al.  Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus. , 2009, Biomaterials.

[130]  A. Artemenko,et al.  Poly(ethylene oxide)‐like Plasma Polymers Produced by Plasma‐Assisted Vacuum Evaporation , 2010 .

[131]  J. Lambert,et al.  A Prospective Multicenter Study of the Efficacy and Tolerability of Cryopreserved Allogenic Human Keratinocytes to Treat Venous Leg Ulcers , 2005, The international journal of lower extremity wounds.

[132]  Masayuki Yamato,et al.  Transplantable urothelial cell sheets harvested noninvasively from temperature-responsive culture surfaces by reducing temperature. , 2003, Tissue engineering.

[133]  T. Okano,et al.  Growth factor and matrix molecules preserve cell function on thermally responsive culture surfaces. , 1999, Tissue engineering.

[134]  G. Blunn,et al.  The development of fibronectin-functionalised hydroxyapatite coatings to improve dermal fibroblast attachment in vitro. , 2012, The Journal of bone and joint surgery. British volume.

[135]  M. Ferguson,et al.  Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration , 2007, Journal of The Royal Society Interface.

[136]  T. Okano,et al.  Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells , 1990 .

[137]  R. M. Warner,et al.  Treatment of burns and chronic wounds using a new cell transfer dressing for delivery of autologous keratinocytes , 2005, European Journal of Plastic Surgery.

[138]  H Green,et al.  Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[139]  J. Mulliken,et al.  GRAFTING OF BURNS WITH CULTURED EPITHELIUM PREPARED FROM AUTOLOGOUS EPIDERMAL CELLS , 1981, The Lancet.

[140]  Guihong Li,et al.  Bone marrow mesenchymal stem cell therapy in ischemic stroke: mechanisms of action and treatment optimization strategies , 2016, Neural regeneration research.

[141]  R. Short,et al.  Comparison of proliferation and growth of human keratinocytes on plasma copolymers of acrylic acid/1,7-octadiene and self-assembled monolayers. , 1999 .