Cell Culture Models and Methods

This chapter reviews methods to harvest and culture several different cell systems commonly employed in cardiac electrophysiology research. The cell systems covered fall into three main categories: (1) primary cell culture; (2) cell lines; and (3) stem cell-derived cardiomyocytes. Each cell system has its own set of advantages and disadvantages. Often the best choice depends on the question an investigator is attempting to answer and the specific experiments involved. The information provided in this chapter is intended to help investigators make such decisions.

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