Novel Resorbable and Osteoconductive Calcium Silicophosphate Scaffold Induced Bone Formation

This aim of this research was to develop a novel ceramic scaffold to evaluate the response of bone after ceramic implantation in New Zealand (NZ) rabbits. Ceramics were prepared by the polymer replication method and inserted into NZ rabbits. Macroporous scaffolds with interconnected round-shaped pores (0.5-1.5 mm = were prepared). The scaffold acted as a physical support where cells with osteoblastic capability were found to migrate, develop processes, and newly immature and mature bone tissue colonized on the surface (initially) and in the material's interior. The new ceramic induced about 62.18% ± 2.28% of new bone and almost complete degradation after six healing months. An elemental analysis showed that the gradual diffusion of Ca and Si ions from scaffolds into newly formed bone formed part of the biomaterial's resorption process. Histological and radiological studies demonstrated that this porous ceramic scaffold showed biocompatibility and excellent osteointegration and osteoinductive capacity, with no interposition of fibrous tissue between the implanted material and the hematopoietic bone marrow interphase, nor any immune response after six months of implantation. No histological changes were observed in the various organs studied (para-aortic lymph nodes, liver, kidney and lung) as a result of degradation products being released.

[1]  Julian R. Jones.  New trends in bioactive scaffolds: The importance of nanostructure , 2009 .

[2]  A. Piattelli,et al.  Maxillary sinus augmentation with Bio-Oss particles: a light, scanning, and transmission electron microscopy study in man. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[3]  J. K. Leach,et al.  Proangiogenic Potential of a Collagen/Bioactive Glass Substrate , 2008, Pharmaceutical Research.

[4]  L. Meseguer-Olmo,et al.  Morphological and Structural Study of a Novel Porous Nurse’s A Ceramic with Osteoconductive Properties for Tissue Engineering , 2016, Materials.

[5]  C. Baudín,et al.  Nurse′s A‐Phase: Synthesis and Characterization in the Binary System Ca2SiO4–Ca3(PO4)2 , 2015 .

[6]  C. Baudín,et al.  Bone neoformation of a novel porous resorbable Si-Ca-P-based ceramic with osteoconductive properties: physical and mechanical characterization, histological and histomorphometric study. , 2016, Clinical oral implants research.

[7]  I. M. Martínez,et al.  New block graft of α-TCP with silicon in critical size defects in rabbits: Chemical characterization, histological, histomorphometric and micro-CT study , 2012 .

[8]  L. Meseguer-Olmo,et al.  Revising the Subsystem Nurse’s A-Phase-Silicocarnotite within the System Ca3(PO4)2–Ca2SiO4 , 2016, Materials.

[9]  F. Nielsen,et al.  Dietary silicon and arginine affect mineral element composition of rat femur and vertebra , 2002, Biological Trace Element Research.

[10]  Icola,et al.  Resorbable Glass-Ceramic Phosphate-Based Scaffolds for Bone Tissue Engineering: Synthesis, Properties and In Vitro Effects on Human Marrow Stromal Cells , 2016 .

[11]  Cato T Laurencin,et al.  Three-dimensional, bioactive, biodegradable, polymer-bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro. , 2003, Journal of biomedical materials research. Part A.

[12]  W. Harris,et al.  Bone ingrowth into porous coated canine total hip replacements. Quantification by backscattered scanning electron microscopy and image analysis. , 1989, Scanning microscopy.

[13]  Larry L Hench,et al.  Third-Generation Biomedical Materials , 2002, Science.

[14]  Francesco Brun,et al.  Microstructural characterization and in vitro bioactivity of porous glass-ceramic scaffolds for bone regeneration by synchrotron radiation X-ray microtomography , 2013 .

[15]  Luis Meseguer-Olmo,et al.  αTCP ceramic doped with dicalcium silicate for bone regeneration applications prepared by powder metallurgy method: in vitro and in vivo studies. , 2013, Journal of biomedical materials research. Part A.

[16]  Marcus Abboud,et al.  Physical properties, mechanical behavior, and electron microscopy study of a new α-TCP block graft with silicon in an animal model. , 2012, Journal of biomedical materials research. Part A.

[17]  G. Nagelschmidt.  179. A new calcium silicophosphate , 1937 .

[18]  Silvia Caddeo,et al.  Using porous bioceramic scaffolds to model healthy and osteoporotic bone , 2016 .

[19]  L. Meseguer-Olmo,et al.  Bioeutectic® Ceramics for Biomedical Application Obtained by Laser Floating Zone Method. In vivo Evaluation , 2014, Materials.

[20]  J. Calvo-Guirado,et al.  Biodegradation Process of α-Tricalcium Phosphate and α-Tricalcium Phosphate Solid Solution Bioceramics In Vivo: A Comparative Study , 2013, Microscopy and Microanalysis.

[21]  J. Gmiński,et al.  The action of excessive, inorganic silicon (Si) on the mineral metabolism of calcium (Ca) and magnesium (Mg) , 1993, Biological Trace Element Research.

[22]  Marcus Abboud,et al.  Comparison of three hydroxyapatite/β-tricalcium phosphate/collagen ceramic scaffolds: an in vivo study. , 2014, Journal of biomedical materials research. Part A.

[23]  Patricia Mazón,et al.  Phase transitions in single phase Si–Ca–P-based ceramic under thermal treatment , 2015 .

[24]  K. Bachus,et al.  Influence of mineral content and composition on graylevels in backscattered electron images of bone. , 1993, Journal of biomedical materials research.

[25]  P. N. Aza,et al.  New evaluation of the in vitro response of silicocarnotite monophasic material , 2015 .

[26]  C. Ascaso,et al.  Advances in the ultrastructural study of the implant-bone interface by backscattered electron imaging. , 2008, Micron.

[27]  M. Sainz,et al.  Single-phase silicocarnotite synthesis in the subsystem Ca3(PO4)2–Ca2SiO4 , 2014 .

[28]  Patricia Mazón,et al.  Material processing of a new calcium silicophosphate ceramic , 2016 .

[29]  K. Bachus,et al.  The meaning of graylevels in backscattered electron images of bone. , 1993, Journal of biomedical materials research.

[30]  Dietmar Werner Hutmacher,et al.  State of the art and future directions of scaffold‐based bone engineering from a biomaterials perspective , 2007, Journal of tissue engineering and regenerative medicine.