Differential pulse adsorptive stripping voltammetric determination of methotrexate using a functionalized carbon nanotubes-modified glassy carbon electrode

AbstractA glassy carbon electrode (GC) containing multiwalled functionalized carbon nanotubes (MWCNTs) immobilized within a dihexadecylhydrogenphosphate film (DHP) is proposed as a nanostructured platform for determination of methotrexate (MTX) concentration (a drug used in cancer treatment) using differential pulse adsorptive stripping voltammetry (DPAdSV). The voltammograms for a MTX solution using MWCNTs-DHP/GC electrode presented an oxidation peak potential at 0.98 V vs. Ag/AgCl (3.0 mol L−1 KCl) in a 0.1 mol L−1 sulphuric acid. The apparent heterogeneous electron transfer rate constant of 0.46 s−1 was calculated. The recovery area of 2.62×10−9 mol cm2 was also obtained. Under the optimal experimental conditions, the analytical curve was linear in the MTX concentration range from 5.0×10−8 to 5.0×10−6 mol L−1, with a detection limit of 3.3×10−8 mol L−1. The MWCNTs-DHP/GC electrode can be easily prepared and was applied for the determination of MTX in pharmaceutical formulations, with results similar to those obtained using a high-performance liquid chromatography comparative method.

[1]  J. A. Ardila,et al.  Determination of gemfibrozil in pharmaceutical and urine samples by square-wave adsorptive stripping voltammetry using a glassy carbon electrode modified with multi-walled carbon nanotubes within a dihexadecyl hydrogen phosphate film , 2013 .

[2]  B. Ye,et al.  Sensitive determination of methotrexate at nano-Au self-assembled monolayer modified electrode , 2012 .

[3]  Hong Ding,et al.  Electrochemical Determination of Methotrexate at a Disposable Screen-Printed Electrode and Its Application Studies , 2012 .

[4]  G. Rivas,et al.  Dispersion of multi-wall carbon nanotubes in glucose oxidase: Characterization and analytical applications for glucose biosensing , 2012 .

[5]  L. Mattoso,et al.  Differential pulse voltammetric determination of ciprofibrate in pharmaceutical formulations using a glassy carbon electrode modified with functionalized carbon nanotubes within a poly(allylamine hydrochloride) film , 2012 .

[6]  S. Jimi,et al.  Simple and sensitive HPLC method for the fluorometric determination of methotrexate and its major metabolites in human plasma by post-column photochemical reaction. , 2012, Biomedical chromatography : BMC.

[7]  Rasa Pauliukaite,et al.  Direct electron transfer of glucose oxidase at glassy carbon electrode modified with functionalized carbon nanotubes within a dihexadecylphosphate film , 2011 .

[8]  Edenir Rodrigues Pereira-Filho,et al.  Development of a carbon nanotubes paste electrode modified with crosslinked chitosan for cadmium(II) and mercury(II) determination , 2011 .

[9]  T. Navrátil,et al.  Voltammetric Behavior of Methotrexate Using Mercury Meniscus Modified Silver Solid Amalgam Electrode , 2011 .

[10]  J. Riu,et al.  Electrochemical sensing based on carbon nanotubes , 2010 .

[11]  Xin Wang,et al.  Influence of carboxylic functionalization of MWCNTs on the thermal properties of MWCNTs/DGEBA/EMI-2,4 nanocomposites , 2009 .

[12]  Guoquan Liu,et al.  Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer. , 2009, Journal of chromatography. A.

[13]  Ronaldo C. Faria,et al.  Anodic stripping voltammetric determination of copper(II) using a functionalized carbon nanotubes paste electrode modified with crosslinked chitosan , 2009 .

[14]  Yunhua Wu.  Nano-TiO2/dihexadecylphosphate based electrochemical sensor for sensitive determination of pentachlorophenol , 2009 .

[15]  B. Ye,et al.  Anodic voltammetric behaviors of methotrexate at a glassy carbon electrode and its determination in spiked human urine , 2007 .

[16]  Yunhua Wu,et al.  Direct electrochemistry of glucose oxidase in a colloid Au-dihexadecylphosphate composite film and its application to develop a glucose biosensor. , 2007, Bioelectrochemistry.

[17]  P. Yáñez‐Sedeño,et al.  Determination of beta-carboline alkaloids in foods and beverages by high-performance liquid chromatography with electrochemical detection at a glassy carbon electrode modified with carbon nanotubes. , 2007, Analytica chimica acta.

[18]  Ying Wang,et al.  A highly sensitive hydrogen peroxide amperometric sensor based on MnO2 nanoparticles and dihexadecyl hydrogen phosphate composite film , 2006 .

[19]  A. Rinzler,et al.  Carbon nanotube actuators , 1999, Science.

[20]  P. Adamson,et al.  Dihydrofolate reductase enzyme inhibition assay for plasma methotrexate determination using a 96-well microplate reader. , 1999, Clinical chemistry.

[21]  P. Sadler,et al.  Immobilization and Visualization of DNA and Proteins on Carbon Nanotubes , 1998 .

[22]  Young Hee Lee,et al.  Crystalline Ropes of Metallic Carbon Nanotubes , 1996, Science.

[23]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[24]  B. McDuffie,et al.  Diffusion coefficients of ferri- and ferrocyanide ions in aqueous media, using twin-electrode thin-layer electrochemistry , 1970 .

[25]  W. Eichelberger.  THE ASTRONOMICAL AND ASTROPHYSICAL SOCIETY OF AMERICA. , 1902, Science.

[26]  C. Pollard.  SOME STRANGE PRACTICES IN PLANT NAMING. , 1901, Science.