Assay and Storage Conditions Affect Yield of Salt Soluble Protein from Muscle

Pork and beef muscles were used to evaluate assay and storage condition effects on yields of salt soluble protein (SSP). Assay conditions included pH (5.5, 6.0 or 6.5), extraction volume (6, 9, 12 or 15 X sample weight), homogenization time (30, 60, 90 or 120 set) and centrifugal force (2500, 4000 or 5500 ×g). Storage conditions included postmortem aging (4°C for 0, 7 or 14 days) and freezing conditions (rapid freezing, slow freezing, and freezing-thawing-freezing). Highest yield of SSP was obtained using pH 6.0, extraction volume 15 X sample weight, centrifugation at 4000 ×g and homogenization time 90 to 120 set for both pork and beef. Freezing conditions did not affect measurement of SSP. Aging beef for 7 and 14 days postmortem reduced the yield of SSP; however, such differences were not observed for pork.

[1]  E. Foegeding.  Functional Properties of Turkey Salt‐Soluble Proteins , 1987 .

[2]  M. Añón,et al.  Effect of frozen storage on protein denaturation in bovine muscle. , 1986 .

[3]  W. Powrie,et al.  Chemical deterioration of frozen bovine muscle at -4°C. , 1968 .

[4]  J. Regenstein,et al.  FACTORS AFFECTING THE SODIUM CHLORIDE EXTRACTABILITY OF MUSCLE PROTEINS FROM CHICKEN BREAST, TROUT WHITE AND LOBSTER TAIL MUSCLES , 1980 .

[5]  F. E. Cunningham,et al.  Salt-Soluble Proteins of Poultry Meat 1. Composition and Emulsifying Capacit , 1971 .

[6]  A. Asghar,et al.  Protein extractability and thermal gel formability of myofibrils isolated from skeletal and cardiac muscles at different post-mortem periods , 1992 .

[7]  D. Mulvihill,et al.  Surface properties of muscle protein extracts. , 1989, Meat science.

[8]  T. R. Dutson,et al.  Processing Properties of Pork as Affected by Electrical Stimulation, Post-Slaughter Chilling and Muscle Group , 1983 .

[9]  L. Berg,et al.  Effects of Frozen Storage on Chicken Muscle Proteins , 1963 .

[10]  K. Nath,et al.  PALATABILITY AND OTHER CHARACTERISTICS OF REPEATEDLY REFROZEN CHICKEN BROILERS , 1976 .

[11]  A. Asghar,et al.  Functionality of muscle proteins in gelation mechanisms of structured meat products. , 1990, Critical reviews in food science and nutrition.

[12]  A. Booren,et al.  Effect of reduced sodium chloride concentration and tetrasodium pyrophosphate on pH, water-holding capacity and extractable protein of prerigor and postrigor ground beef. , 1991, Meat Science.

[13]  J. Acton.  THE EFFECT OF MEAT PARTICLE SIZE ON EXTRACTABLE PROTEIN, COOKING LOSS AND BINDING STRENGTH IN CHICKEN LOAVES , 1972 .

[14]  H. Turgut.  Emulsifying Capacity and Stability of Goat, Waterbuffalo, Sheep and Cattle Muscle Proteins , 1984 .

[15]  S. Hara,et al.  Physicochemical Properties and Heat-induced Gelling of Cardiac Myosin in Model System , 1985 .

[16]  J. Anderson,et al.  EXTRACTABLE‐EMULSIFYING CAPACITY OF HAND AND MECHANICALLY‐DEBONED MUTTON , 1974 .

[17]  Y. Xiong,et al.  Changes in protein solubility and gelation properties of chicken myofibrils during storage. , 1989 .

[18]  F. C. Parrish,et al.  MOLECULAR CHANGES IN THE SALT‐SOLUBLE MYOFIBRILLAR PROTEINS OF BOVINE MUSCLE , 1978 .

[19]  E. Li-Chan,et al.  Hydrophobicity and Solubility of Meat Proteins and Their Relationship to Emulsifying Properties , 1984 .

[20]  R. H. Locker,et al.  The effect of repeated freeze–thaw cycles on tenderness and cooking loss in beef , 1973 .

[21]  S. Palumbo,et al.  EFFECTS OF FROZEN STORAGE ON FUNCTIONALITY OF MEAT FOR PROCESSING , 1980 .

[22]  J. Acton.  EFFECT OF HEAT PROCESSING ON EXTRACTABILITY OF SALT‐SOLUBLE PROTEIN, TISSUE BINDING STRENGTH AND COOKING LOSS IN POULTRY MEAT LOAVES , 1972 .

[23]  E. Li-Chan,et al.  Physicochemical and Functional Properties of Salt-Extractable Proteins From Chicken Breast Muscle Deboned After Different Post-mortem Holding Times1 , 1986 .

[24]  C. G. Haugh,et al.  PHYSICAL AND CHEMICAL INFLUENCES ON MEAT EMULSION STABILITY IN A MODEL EMULSITATOR , 1977 .