Transcriptional pattern of Escherichia coli ihfB (himD) gene expression.

Integration host factor (IHF) is a small heterodimer containing subunits encoded by the unlinked ihfA (himA) and ihfB (himD, hip) genes. The transcriptional pattern of ihfB expression in the logarithmic and stationary growth phases was investigated. The ihfB gene is expressed as both monocistronic and polycistronic (hybridizing also to an internal rpsA probe) transcript. The intensity of the polycistronic transcripts, initiated upstream of rpsA, decreased sharply upon growth cessation. In contrast, expression of the monocistronic ihfB transcript strongly increased when cells entered stationary growth phase. The observed growth rate-dependent regulation of the transcription of these transcripts is in agreement with the previously published data about the regulation of the rpsA and ihfB promoters (Pedersen et al., 1984; Aviv et al., 1994).

[1]  M. Grunberg‐Manago,et al.  Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon. , 1985, Journal of molecular biology.

[2]  A. Sirko,et al.  The role of integration host factor In gene expression in Escherichia coli , 1992, Molecular microbiology.

[3]  H. Nash,et al.  Characterization of a set of integration host factor mutants deficient for DNA binding. , 1993, Journal of Molecular Biology.

[4]  S. Pedersen,et al.  The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli , 1995, Journal of bacteriology.

[5]  Y. Mechulam,et al.  Sequence of the Escherichia coli pheST operon and identification of the himA gene , 1985, Journal of bacteriology.

[6]  Andrew Wright,et al.  Transcriptional antitermination in the bgl operon of E. coli is modulated by a specific RNA binding protein , 1990, Cell.

[7]  H. Aiba,et al.  Evidence for two functional gal promoters in intact Escherichia coli cells. , 1981, The Journal of biological chemistry.

[8]  L. Lindahl,et al.  Ribosomal protein L4 stimulates in vitro termination of transcription at a NusA-dependent terminator in the S10 operon leader. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[9]  E. Brody,et al.  Prediction of rho-independent Escherichia coli transcription terminators. A statistical analysis of their RNA stem-loop structures. , 1990 .

[10]  Y. Mechulam,et al.  Dual level control of the Escherichia coli pheST-himA operon expression. tRNA(Phe)-dependent attenuation and transcriptional operator-repressor control by himA and the SOS network. , 1987, Journal of molecular biology.

[11]  Gideon Schreiber,et al.  Expression of the genes coding for the Escherichia coli integration host factor are controlled by growth phase, rpoS, ppGpp and by autoregulation , 1994, Molecular microbiology.

[12]  R. Weisberg,et al.  Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor. , 1985, Journal of molecular biology.

[13]  C. Yanofsky,et al.  Reconstitution of Bacillus subtilis trp attenuation in vitro with TRAP, the trp RNA-binding attenuation protein. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[14]  I. Schwartz,et al.  Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli. , 1988, Gene.

[15]  S. Cohen,et al.  Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. Friedman,et al.  Integration host factor: A protein for all reasons , 1988, Cell.

[17]  D Roberts,et al.  Growth phase variation of integration host factor level in Escherichia coli , 1994, Journal of bacteriology.

[18]  Nora Goosen,et al.  The regulation of transcription initiation by integration host factor , 1995, Molecular microbiology.