Gastrointestinal Pathogenicity of Adenoviruses and Reoviruses Isolated from Broiler Chickens in Alabama

Adenoviruses and reoviruses isolated from commercial broiler chickens were evaluated for gastrointestinal pathogenicity in specific-pathogen-free Leghorn chickens. The viruses were originally isolated from either the proventriculus or a gastrointestinal pool of tissues of broiler chickens with proventriculitis or enteritis. Isolates were cloned by terminal dilution. Day-old chickens were inoculated by oral and ocular routes with undiluted tissue culture fluids (titers of 102-104 TCID50/ml) and then examined at necropsy on days 5, 10, and 15 postinoculation. Chickens in all virus groups (but not the control group) developed wet, unformed fecal droppings that persisted for the duration of the study. Mild lesions occurred in reovirus-inoculated chickens and included hyperplasia of lymphocyte aggregates in various organs and mild gizzard erosions. Chickens inoculated with adenovirus isolates developed marked gizzard erosions and necrotizing pancreatitis as well as mild proventriculitis. Intranuclear viral inclusion bodies occurred in gizzard epithelium and pancreatic acinar cells at the sites of lesions. Lymphocytic atrophy occurred in the bursa of Fabricius. Respective viruses were reisolated from proventriculus and duodenum collected from chickens of each group; no viruses were isolated from controls. Under the conditions of this study, adenovirus isolates were more pathogenic than the reovirus isolates in the digestive system.

[1]  M. Goodwin.  Adenovirus inclusion body ventriculitis in chickens and captive bobwhite quail (Colinus virginianus). , 1993, Avian diseases.

[2]  K. Nakamura,et al.  Necrotizing pancreatitis and gizzard erosion associated with adenovirus infection in chickens. , 1993, Avian diseases.

[3]  M. Goodwin,et al.  Enteritis (so-called runting stunting syndrome) in Georgia broiler chicks. , 1993, Avian diseases.

[4]  D. L. Hill,et al.  Multisystemic adenovirus infection in broiler chicks with hypoglycemia and spiking mortality. , 1993, Avian diseases.

[5]  R. De,et al.  In vitro and in vivo characterization of avian reoviruses. III. Host factors affecting virulence and persistence. , 1989, Avian diseases.

[6]  M. D. Ruff,et al.  In vitro and in vivo characterization of avian reoviruses. II. Clinical evaluation of chickens infected with two avian reovirus pathotypes. , 1989, Avian diseases.

[7]  A. Margolin,et al.  In vitro and in vivo characterization of avian reoviruses. I. Pathogenicity and antigenic relatedness of several avian reovirus isolates. , 1989, Avian diseases.

[8]  J. Rosenberger,et al.  In vitro and in vivo characterization of avian reoviruses. III. Host factors affecting virulence and persistence. , 1989, Avian diseases.

[9]  R. Harvey,et al.  Response of growing Leghorn chicks to deoxynivalenol-contaminated wheat. , 1988, Poultry science.

[10]  O. Fossum,et al.  Gizzard erosions as a cause of mortality in White Leghorn chickens. , 1988, Avian pathology : journal of the W.V.P.A.

[11]  R. Harvey,et al.  Effects of feeding deoxynivalenol (DON, vomitoxin)-contaminated wheat to female White Leghorn chickens from day old through egg production. , 1987, Poultry science.

[12]  Stuart Bp,et al.  Proventricular hyperplasia (malabsorption syndrome) in broiler chickens. , 1986 .

[13]  G. Ware,et al.  Gas chromatographic determination of deoxynivalenol in wheat with electron capture detection. , 1984, Journal - Association of Official Analytical Chemists.

[14]  J. Devries,et al.  Rapid determination of deoxynivalenol (vomitoxin) by liquid chromatography using modified Romer column cleanup. , 1984, Journal - Association of Official Analytical Chemists.

[15]  K. Mori,et al.  Gizzerosine, a New Toxic Substance in Fish Meal, Causes Severe Gizzard Erosion in Chicks , 1983 .

[16]  R. J. Cole,et al.  Cyclopiazonic acid production by Aspergillus flavus and its effects on broiler chickens , 1983, Applied and environmental microbiology.

[17]  S. Kleven,et al.  Identification and serological differentiation of several reovirus strains isolated from chickens with suspected malabsorption syndrome. , 1983, Avian diseases.

[18]  N. Daghir,et al.  Vitamin B6 in the etiology of gizzard erosion in growing chickens. , 1981, Poultry science.

[19]  W. B. Gross,et al.  Some effects of feeding deoxycorticosterone to chickens. , 1980, Poultry science.

[20]  L. D. Olson,et al.  Influence of Pasteurella multocida and high and low environmental temperatures on adrenals and bursa of Fabricius in turkeys. , 1980, Avian diseases.

[21]  D. Patterson,et al.  Mycotoxins in animal feedstuffs: sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin. , 1979, Journal - Association of Official Analytical Chemists.

[22]  J. Giambrone,et al.  Effect of tenuazonic acid on young chickens. , 1978, Poultry science.

[23]  M. C. Bowman,et al.  Trace analysis of zearalenone and/or zearalanol in animal chow by high pressure liquid chromatography and gas-liquid chromatography. , 1977, Journal - Association of Official Analytical Chemists.

[24]  King Dj,et al.  Effect of maternal antibody on experimental infections of chickens with a type-8 avian adenovirus. , 1977 .

[25]  D. King,et al.  Effect of maternal antibody on experimental infections of chickens with a type-8 avian adenovirus. , 1977, Avian diseases.

[26]  E. Harry,et al.  The effect of orally administered histamine on the weight gain and development of gizzard lesions in chicks , 1976, Veterinary Record.

[27]  E. Harry,et al.  The role of histamine and fish meal in the incidence of gizzard erosion and pro-ventricular abnormalities in the fowl. , 1975, British poultry science.