High-Resolution, In Vivo Magnetic Resonance Imaging of Drosophila at 18.8 Tesla

High resolution MRI of live Drosophila was performed at 18.8 Tesla, with a field of view less than 5 mm, and administration of manganese or gadolinium-based contrast agents. This study demonstrates the feasibility of MR methods for imaging the fruit fly Drosophila with an NMR spectrometer, at a resolution relevant for undertaking future studies of the Drosophila brain and other organs. The fruit fly has long been a principal model organism for elucidating biology and disease, but without capabilities like those of MRI. This feasibility marks progress toward the development of new in vivo research approaches in Drosophila without the requirement for light transparency or destructive assays.

[1]  S J Blackband,et al.  NMR spectroscopy of single neurons , 2000, Magnetic resonance in medicine.

[2]  C. Cheong,et al.  One micrometer resolution NMR microscopy. , 2001, Journal of magnetic resonance.

[3]  M Gribskov,et al.  A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster. , 2001, Genome research.

[4]  B. S. Baker,et al.  Gene Expression During the Life Cycle of Drosophila melanogaster , 2002, Science.

[5]  A. Jasanoff,et al.  In vivo magnetic resonance microscopy of brain structure in unanesthetized flies. , 2002, Journal of magnetic resonance.

[6]  T. Wenseleers,et al.  Magnetic resonance imaging in entomology: a critical review , 2003, Journal of insect science.

[7]  H. Manev,et al.  Techniques: fruit flies as models for neuropharmacological research. , 2003, Trends in pharmacological sciences.

[8]  James R. Knight,et al.  A Protein Interaction Map of Drosophila melanogaster , 2003, Science.

[9]  Liliane Schoofs,et al.  Peptidomics in Drosophila melanogaster. , 2003, Briefings in functional genomics & proteomics.

[10]  K. Lilley,et al.  Proteomics in Drosophila melanogaster. , 2003, Briefings in functional genomics & proteomics.

[11]  U. Theopold,et al.  Proteomics of the Drosophila immune response. , 2004, Trends in biotechnology.

[12]  Wilson Fong.  Handbook of MRI Pulse Sequences , 2005 .

[13]  A. Haase,et al.  NMR Imaging of the honeybee brain , 2004, Journal of insect science.

[14]  Jun Shen,et al.  A new strategy for in vivo spectral editing. Application to GABA editing using selective homonuclear polarization transfer spectroscopy. , 2004, Journal of magnetic resonance.

[15]  INTRODUCTION TO GRADIENTS , 2004 .

[16]  Xiangyi Lu,et al.  Drosophila nutrigenomics can provide clues to human gene-nutrient interactions. , 2005, Annual review of nutrition.

[17]  Volker Dötsch,et al.  In‐Cell NMR Spectroscopy , 2005, Chembiochem : a European journal of chemical biology.

[18]  T. Kaufman,et al.  Mapping the proteome of Drosophila melanogaster: analysis of embryos and adult heads by LC-IMS-MS methods. , 2005, Journal of proteome research.

[19]  Ethan Bier,et al.  Drosophila, the golden bug, emerges as a tool for human genetics , 2005, Nature Reviews Genetics.

[20]  Scott E Fraser,et al.  Magnetic resonance microscopy: recent advances and applications. , 2005, Current opinion in biotechnology.

[21]  Gerhard Wagner,et al.  Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes , 2006, Proceedings of the National Academy of Sciences.

[22]  D. Mietchen,et al.  Subcellular in vivo 1H MR spectroscopy of Xenopus laevis oocytes. , 2006, Biophysical journal.

[23]  John H Krystal,et al.  MR spectroscopy: its potential role for drug development for the treatment of psychiatric diseases , 2006, NMR in biomedicine.

[24]  R. Gillies,et al.  Redox‐sensitive contrast agents for MRI based on reversible binding of thiols to serum albumin , 2006, Magnetic resonance in medicine.

[25]  N. Raghunand.  Tissue pH measurement by magnetic resonance spectroscopy and imaging. , 2006, Methods in molecular medicine.

[26]  Piotr Walczak,et al.  Artificial reporter gene providing MRI contrast based on proton exchange , 2007, Nature Biotechnology.

[27]  Kristina D. Micheva,et al.  Array Tomography: A New Tool for Imaging the Molecular Architecture and Ultrastructure of Neural Circuits , 2007, Neuron.

[28]  T. Kaufman,et al.  Protein expression in a Drosophila model of Parkinson's disease. , 2007, Journal of proteome research.

[29]  Stephen J. Smith,et al.  Array Tomography: A New Tool for Imaging the Molecular Architecture and Ultrastructure of Neural Circuits , 2007, Neuron.

[30]  Imaging the functional plasticity of identified cell types in visual cortex , 2010 .