A glucan 1,3-β-glucosidase (EC 3.2.1.58) and an N-acetyl-β-glucosaminidase (EC 3.2.1.30) were purified to homogeneity from the culture filtrate of Tricloderma harzianum strain PI. The molecular masses and the pIs were 78 kDa and 6.2, respectively, for the glucan 1,3-β-glucosidase and 72 kDa and 4.6, respectively, for the N-acetyl-β-glucosaminidase. The glucan 1,3-β-glucosidase and the N-acetyl-β-glucosaminidase were tested against Botrytis cinerea, and their antifungal activity was compared with that obtained for an endochitinase and a chitin 1,4-β-chitobiosidase also purified from T. harzianun strain P1. The four cell wall-degrading enzymes were also tested as mixtures containing two, three, or all four proteins in all possible combinations [...]