Distinct isolates of uropathogenic Escherichia coli differentially affect human sperm parameters in vitro

Sperm motility and vitality are decreased in male genital tract infection. Uropathogenic Escherichia coli (UPEC) are frequently associated with sperm parameter loss, but there are no reports to date regarding the effects of different E. coli isolates on human spermatozoa. The aim of this work was to compare the effect in vitro of different E. coli isolates on human sperm parameters. Normal spermatozoa were incubated with E. coli isolated from nine men with urinary tract infection. After 1 h of incubation, sperm motility, vitality and mitochondrial membrane potential (ΔΨm) were measured. The E. coli isolates were serotyped with specific antisera. Sperm motility was decreased with five of nine E. coli isolates. Two UPEC were typed as O6 strains, and they did not decrease sperm motility in the same experimental conditions as the other five isolates, despite the described high pathogenicity of the O6 strain in urogenital infections. Neither UPEC analysed affected vitality or ΔΨm. UPEC isolates were shown to be heterogeneous in their effects, suggesting the need to characterise the pattern defining the pathogenicity of E. coli on human spermatozoa.

[1]  M. Piasecka,et al.  Membrane stability and mitochondrial activity of human‐ejaculated spermatozoa during in vitro experimental infection with Escherichia coli, Staphylococcus haemolyticus and Bacteroides ureolyticus , 2012, Andrologia.

[2]  J. Risopatrón,et al.  Effect of Escherichia coli and its soluble factors on mitochondrial membrane potential, phosphatidylserine translocation, viability, and motility of human spermatozoa. , 2010, Fertility and sterility.

[3]  Organización Mundial de la Salud.  WHO laboratory manual for the examination and processing of human semen , 2010 .

[4]  R. Sánchez,et al.  Integrity of mitochondrial membrane potential reflects human sperm quality , 2009, Andrologia.

[5]  G. Collodel,et al.  The presence of bacteria species in semen and sperm quality , 2008, Journal of Assisted Reproduction and Genetics.

[6]  E. Bingen,et al.  Combined multilocus sequence typing and O serogrouping distinguishes Escherichia coli subtypes associated with infant urosepsis and/or meningitis. , 2007, The Journal of infectious diseases.

[7]  Y. Yılmaz,et al.  Sperm motility changes after coincubation with various uropathogenic microorganisms: an in vitro experimental study , 2007, International Urology and Nephrology.

[8]  C. Buchrieser,et al.  How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains , 2006, Proceedings of the National Academy of Sciences.

[9]  M. Kurpisz,et al.  Bacterial infection and semen quality. , 2005, Journal of reproductive immunology.

[10]  J. Hacker,et al.  Properties ofEscherichia coli strains of serotype O6 , 1995, Infection.

[11]  F. Galdiero,et al.  The action of LPS porins and peptidoglycan fragments on human spermatozoa , 2005, Infection.

[12]  C. Jantos,et al.  Chronic prostatitis: A thorough search for etiologically involved microorganisms in 1,461 patients , 2005, Infection.

[13]  T. Diemer,et al.  Influence of autogenous leucocytes and Escherichia coli on sperm motility parameters in vitro , 2003, Andrologia.

[14]  P. Marchetti,et al.  Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm. , 2002, Human reproduction.

[15]  F. Mayer,et al.  Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis. , 2000, International journal of andrology.

[16]  T. Diemer,et al.  Influence of different uropathogenic microorganisms on human sperm motility parameters in an in vitro experiment , 1998, Andrologia.

[17]  Y. Takeda,et al.  Escherichia coli Virulence Factors and Serotypes in Acute Bacterial Prostatitis , 1997, International journal of urology : official journal of the Japanese Urological Association.

[18]  F. Mayer,et al.  Influence of Escherichia coli on motility parameters of human spermatozoa in vitro. , 1996, International journal of andrology.

[19]  A. Sheth,et al.  Alteration of semen characteristics and regulatory factors in human semen with bacterial infection. , 1994, Archives of andrology.

[20]  M. Monga,et al.  Spermagglutination by bacteria: receptor-specific interactions. , 1994, Journal of andrology.

[21]  J. R. Johnson,et al.  O, K, and H antigens predict virulence factors, carboxylesterase B pattern, antimicrobial resistance, and host compromise among Escherichia coli strains causing urosepsis. , 1994, The Journal of infectious diseases.

[22]  W. Stolz,et al.  Adherence of Escherichia coli to sperm: a mannose mediated phenomenon leading to agglutination of sperm and E. coli. , 1993, Fertility and sterility.

[23]  Y. Nitzan,et al.  Virulence characteristics of male genital tract Escherichia coll isolated from semen of suspected infertile men , 2009, Andrologia.

[24]  C. Jantos,et al.  Semen parameters in men with and without proven chronic prostatitis. , 1991, Archives of andrology.

[25]  G. Warnes,et al.  Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. , 1985, Fertility and sterility.

[26]  R. E. Buchanan,et al.  Bergey's Manual of Determinative Bacteriology. , 1975 .

[27]  S. T. Cowan.  Bergey's Manual of Determinative Bacteriology , 1948, Nature.