Variation in the Concentrations of Major Secondary Metabolites in Ginkgo Leaves from Different Geographical Populations

Ginkgo biloba L. is a well-known relict tree species and an important medicinal plant. Ginkgo is rich in secondary metabolites (SMs), mainly including flavonoids, lactones, and ginkgolic acid. The aim of this study was to determine variations in the concentrations of these SMs in Ginkgo leaves from different geographical populations. The SMs in the leaves of 298 clones from 10 geographical populations grafted under the same conditions were extracted and measured by high performance liquid chromatography (HPLC). The results showed that there were significant differences in concentrations of SMs in leaves from different populations (p < 0.01). The concentrations of both flavonoids and lactones were significantly negatively correlated with that of ginkgolic acid. Altitude and annual rainfall were important factors influencing the concentrations of lactones, and the frost-free period influenced the concentration of isorhamnetin. Population Yingdianjie (YDJ) was ideal for the plantations from which medicinal flavonoids and lactones are extracted, followed by populations Xiaopu (XP), Anlu (AL) and Wuchuan (WC). As variations within each population were found, attention should be paid to selection within populations.

[1]  Christian Gieger,et al.  Genetic variation in metabolic phenotypes: study designs and applications , 2012, Nature Reviews Genetics.

[2]  E. Koch,et al.  In vitro evaluation of the cytotoxic potential of alkylphenols from Ginkgo biloba L. , 2002, Toxicology.

[3]  B. Gosztola,et al.  Variability of total flavonoid and mucilage content of wild growing chamomile ( Matricaria recutita L.) populations , 2016 .

[4]  Beat Meier,et al.  Identification and determination of the flavonoids from Ginkgo biloba by high-performance liquid chromatography , 1992 .

[5]  S. Lai,et al.  Extraction of Flavonoid Glycosides from Ginkgo biloba Leaves and Their Adsorption Separations Using Hydrophobic and Anion-Exchange Membranes , 2003 .

[6]  Yun Li,et al.  Variation in the Growth Traits and Wood Properties of Chinese Fir from Six Provinces of Southern China , 2016 .

[7]  M. Haag-Berrurier,et al.  Seasonal Variations of the Flavonoid Content from Ginkgo biloba Leaves. , 1991, Planta medica.

[8]  Eric Brand,et al.  The formation of daodi medicinal materials. , 2012, Journal of ethnopharmacology.

[9]  R. J. Robbins,et al.  Phenolic acids in foods: an overview of analytical methodology. , 2003, Journal of agricultural and food chemistry.

[10]  E. Schijlen,et al.  Modification of flavonoid biosynthesis in crop plants. , 2004, Phytochemistry.

[11]  Runhui Wang,et al.  Assessing 62 Chinese Fir (Cunninghamia lanceolata) Breeding Parents in a 12-Year Grafted Clone Test , 2015 .

[12]  T. A. Beek.  Chemical analysis of Ginkgo biloba leaves and extracts. , 2002 .

[13]  R. Chapman,et al.  An effective identification and quantification method for Ginkgo biloba flavonol glycosides with targeted evaluation of adulterated products. , 2016, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[14]  A. Husband,et al.  Flavonoid compounds in maintenance of prostate health and prevention and treatment of cancer , 2005, Molecular biotechnology.

[15]  M. Elmastaș,et al.  Changes in flavonoid and phenolic acid contents in some Rosa species during ripening. , 2017, Food chemistry.

[16]  C. China Pharmacopoeia,et al.  Pharmacopoeia of the People's Republic of China , 2010 .

[17]  Kelly E Heim,et al.  Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. , 2002, The Journal of nutritional biochemistry.

[18]  M. Reichelt,et al.  Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in Arabidopsis , 2001, Plant Cell.

[19]  N. Luepke,et al.  Evidence for toxic effects of alkylphenols from Ginkgo biloba in the hen's egg test (HET). , 2001, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[20]  P. Proksch,et al.  Protective and detrimental effects of kaempferol in rat H4IIE cells: implication of oxidative stress and apoptosis. , 2005, Toxicology and applied pharmacology.

[21]  Y. Moon,et al.  Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. , 2006, Toxicology in vitro : an international journal published in association with BIBRA.

[22]  Ling Yuan,et al.  Chemotypic and genetic diversity in Epimedium sagittatum from different geographical regions of China. , 2015, Phytochemistry.

[23]  Shaolin Zheng,et al.  Palaeobiology: The missing link in Ginkgo evolution , 2003, Nature.

[24]  Y. Qiu,et al.  Genetic variation of Ginkgo biloba L. (Ginkgoaceae) based on cpDNA PCR-RFLPs: inference of glacial refugia , 2005, Heredity.

[25]  E. Hafez,et al.  Preventive effect of the flavonoid, quercetin, on hepatic cancer in rats via oxidant/antioxidant activity: molecular and histological evidences , 2009, Journal of experimental & clinical cancer research : CR.

[26]  Wei Gong,et al.  Phylogeography of a living fossil: pleistocene glaciations forced Ginkgo biloba L. (Ginkgoaceae) into two refuge areas in China with limited subsequent postglacial expansion. , 2008, Molecular phylogenetics and evolution.

[27]  M. McIntosh,et al.  The relationship between genetic and chemotypic diversity in American ginseng (Panax quinquefolius L.). , 2013, Phytochemistry.

[28]  Xie Bao-dong,et al.  Effects of Light Spectrum and Photoperiod on Contents of Flavonoid and Terpene in Leaves of Ginkgo biloba L. , 2006 .

[29]  O. Krishtal,et al.  Effects of Ginkgo Biloba Extract Constituents on Glycine-Activated Strychnine-Sensitive Receptors in Hippocampal Pyramidal Neurons of the Rat , 2002, Neurophysiology.

[30]  Y. Asakawa,et al.  Antitumor principles from Ginkgo biloba L. , 1987, Chemical & pharmaceutical bulletin.

[31]  Guiyou Zhang,et al.  Effects of Ginkgo biloba extract (EGb 761) on hydroxyl radical-induced thymocyte apoptosis and on age-related thymic atrophy and peripheral immune dysfunctions in mice , 2003, Mechanisms of Ageing and Development.

[32]  J. Brinckmann.  Emerging Importance of Geographical Indications and Designations of Origin – Authenticating Geo‐Authentic Botanicals and Implications for Phytotherapy , 2013, Phytotherapy research : PTR.

[33]  Lian‐Wen Qi,et al.  Combining Metabolic Profiling and Gene Expression Analysis to Reveal the Biosynthesis Site and Transport of Ginkgolides in Ginkgo biloba L. , 2017, Front. Plant Sci..

[34]  Ahmad Esmaillzadeh,et al.  Dietary flavonoid intake and cardiovascular mortality , 2008, British Journal of Nutrition.