Cybernetic Modeling of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Wood with Brettanomyces custersii

The simultaneous saccharification and fermentation (SSF) process consists of concurrent enzymatic saccharification and fermentation. In the present cybernetic model, the saccharification process, which -is based on the modified Michaelis-Menten kinetics and enzyme inhibition kinetics, was combined with the fermentation process, which is based on the Monod equation. The cybernetic modeling approach postulates that cells adapt to utilize the limited resources available to them in an optimal way. The cybernetic modeling was suitable for describing sequential growth on multiple substrates by Brettanomyces custersii, which is a glucose- and cellobiose-fermenting yeast. The proposed model was able to elucidate the SSF process in a systematic manner, and the performance was verified by previously published data.

[1]  D. Kompala,et al.  Cybernetic model of the growth dynamics of Saccharomyces cerevisiae in batch and continuous cultures. , 1999, Journal of biotechnology.

[2]  Y. Koo,et al.  Modeling and simulation of simultaneous saccharification and fermentation of paper mill sludge to lactic acid , 2005 .

[3]  Lee R. Lynd,et al.  Modeling simultaneous saccharification and fermentation of lignocellulose to ethanol in batch and continuous reactors , 1995 .

[4]  Doraiswami Ramkrishna,et al.  Dynamic analysis of the cybernetic model for diauxic growth , 1997 .

[5]  D. Ramkrishna.  A Cybernetic Perspective of Microbial Growth , 1983 .

[6]  Y. Koo,et al.  Model Development for Lactic Acid Fermentation and Parameter Optimization Using Genetic Algorithm , 2004 .

[7]  C. Wyman,et al.  Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process , 1993, Biotechnology and bioengineering.

[8]  Charles E. Wyman,et al.  Mathematical modeling of cellulose conversion to ethanol by the simultaneous saccharification and fermentation process , 1992 .

[9]  Abhijit Anand Namjoshi,et al.  Multiplicity and stability of steady states in continuous bioreactors: dissection of cybernetic models , 2001 .

[10]  Gregory Stephanopoulos,et al.  Foundations of biochemical engineering : kinetics and thermodynamics in biological systems , 1983 .

[11]  G. T. Tsao,et al.  Investigation of bacterial growth on mixed substrates: Experimental evaluation of cybernetic models , 1986, Biotechnology and bioengineering.