Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower

ABSTRACT Orobanche cumana Wallr. (Orobanche cernua Loefl.) causes severe yield losses of confectionary sunflower in China. While germination of O. cumana is stimulated by sesquiterpene lactones (STLs) from host sunflower (Helianthus annuus L.). Dehydrocostus lactone and costunolide isolated from sunflower root exudates are known as STLs to specifically induce O. cumana germination. Two major confectionary sunflower cultivars, SH363 (highly susceptible to O. cumana) and TH33 (resistant to O. cumana), were planted in China. However, STLs in these two sunflower cultivars has remained unknown. To identify STLs from root and exudates of sunflower for better understanding the role of stimulants in parasitic interaction of sunflower and O. cumana, we tested dehydrocostus lactone (DCL) and costunolide (CL) in root and root exudates of susceptible and resistant sunflower cultivars. The stimulant activity of sunflower root exudate and root extract to germination of O. cumana were also determined. Dehydrocostus lactone and costunolide were identified through ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Both DCL and CL were found in root extracts and root exudates in the whole tested time point from two sunflower cultivars. The concentration of dehydrocostus lactone was higher than that of costunolide at the same tested growth stage of each sunflower cultivar. It was observed that higher quantity of dehydrocostus lactone in susceptible cultivar than resistant cultivar of root and root exudates at later tested developmental stages. However, the amount of CL was no significant difference between SH363 and TH33 at all tested stages. The release amount of DCL from susceptible cultivar is 3.7 folds that of resistant cultivar at 28 DAT. These findings suggested that DCL was the one of the major signal compound in these two sunflower cultivars, and lower dehydrocostus lactone might contribute to the resistance of sunflower TH33 to O. cumana.

[1]  O. Spring,et al.  Costunolide Influences Germ Tube Orientation in Sunflower Broomrape – A First Step Toward Understanding Chemotropism , 2021, Frontiers in Plant Science.

[2]  O. Spring.  Sesquiterpene lactones in sunflower oil , 2021 .

[3]  O. Spring,et al.  Spatial and developmental synthesis of endogenous sesquiterpene lactones supports function in growth regulation of sunflower , 2020, Planta.

[4]  H. Jia,et al.  Pharmacokinetics of costunolide and dehydrocostuslactone after oral administration of Radix aucklandiae extract in normal and gastric ulcer rats , 2018, Journal of Asian natural products research.

[5]  Y. Sugimoto,et al.  Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower. , 2014, Phytochemistry.

[6]  O. Spring,et al.  New sesquiterpene lactones from sunflower root exudate as germination stimulants for Orobanche cumana. , 2013, Journal of agricultural and food chemistry.

[7]  D. Joel,et al.  Dehydrocostus lactone is exuded from sunflower roots and stimulates germination of the root parasite Orobanche cumana. , 2011, Phytochemistry.

[8]  Xiaonan Xie,et al.  Strigolactones as Germination Stimulants for Root Parasitic Plants , 2010, Plant & cell physiology.

[9]  Chris Parker,et al.  Observations on the current status of Orobanche and Striga problems worldwide. , 2009, Pest management science.

[10]  D. Rubiales,et al.  Identification of sources of resistance to crenate broomrape (Orobanche crenata) in Spanish lentil (Lens culinaris) germplasm , 2008 .

[11]  Jesús Jorrín,et al.  Pre-haustorial resistance to broomrape (Orobanche cumana) in sunflower (Helianthus annuus): cytochemical studies. , 2006, Journal of experimental botany.

[12]  K. Yoneyama,et al.  Confirmation and Quantification of Strigolactones, Germination Stimulants for Root Parasitic Plants Striga and Orobanche, Produced by Cotton , 2005, Bioscience, biotechnology, and biochemistry.

[13]  P. Labrousse,et al.  Analysis of resistance criteria of sunflower recombined inbred lines against Orobanche cumana Wallr. , 2004 .

[14]  H. Eizenberg,et al.  Resistance to broomrape (Orobanche spp.) in sunflower (Helianthus annuus L.) is temperature dependent. , 2003, Journal of experimental botany.

[15]  P. Labrousse,et al.  Several Mechanisms are Involved in Resistance of Helianthus to Orobanche cumana Wallr. , 2001 .

[16]  L. Garcia-Torres,et al.  Sunflower (Helianthus annuus L.) response to broomrape (Orobanche cernua Loefl.) parasitism: induced synthesis and excretion of 7-hydroxylated simple coumarins. , 2001, Journal of experimental botany.

[17]  J. Fernández-martínez,et al.  Selection of wild and cultivated sunflower for resistance to a new broomrape race that overcomes resistance of the Or5 gene , 2000 .

[18]  Yongqing Ma,et al.  The potential of foxtail millet as a trap crop for sunflower broomrape , 2016, Acta Physiologiae Plantarum.

[19]  Bai Quan-jiang.  The Occurrence and Control Techniques of Sunflower Broomrapes in Inner Mongolia , 2013 .

[20]  W. Peng.  Preliminary Report on Sunflower Broomrape in Shanxi Province , 2003 .