Wiring horseradish peroxidase on gold nanoparticles-based nanostructured polymeric network for the construction of mediatorless hydrogen peroxide biosensor

Abstract Gold electrodes were functionalized with an electropolymerized matrix of Au nanoparticles modified with 2-mercaptoethanesulfonic acid, 3-mercaptophenyl boronic acid and p -aminothiophenol. The resulting nanostructured electroconductive matrix was used as support for the oriented immobilization of horseradish peroxidase to construct a reagentless amperometric biosensor for H 2 O 2 . The electrode, poised at 0.0 mV, exhibited a rapid response within 8 s and a linear calibration range from 5 μM to 1.1 mM H 2 O 2 . The sensitivity of the biosensor was determined as 498 μA/M cm 2 , and its detection limit was 1.5 μM H 2 O 2 at a signal-to-noise ratio of 3. The electrode retained 95% and 72% of its initial activity after 21 and 40 days of storage at 4 °C.

[1]  Jungbae Kim,et al.  High-performance biosensors based on enzyme precipitate coating in gold nanoparticle-conjugated single-walled carbon nanotube network films , 2010 .

[2]  I. Willner,et al.  Imprinted Au‐Nanoparticle Composites for the Ultrasensitive Surface Plasmon Resonance Detection of Hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX) , 2010, Advanced materials.

[3]  Masayuki Nogami,et al.  Novel Electrochemical Interfaces with a Tunable Kinetic Barrier by Self-Assembling Organically Modified Silica Gel and Gold Nanoparticles , 2001 .

[4]  S. Ai,et al.  A novel hydrogen peroxide biosensor based on horseradish peroxidase immobilized on gold nanoparticles-silk fibroin modified glassy carbon electrode and direct electrochemistry of horseradish peroxidase , 2009 .

[5]  J. Švitel,et al.  Composite Biosensor for Sulfite Assay: Use of Water-Insoluble Hexacyanoferrate(III) Salts as Electron-Transfer Mediators , 1998 .

[6]  Lixian Sun,et al.  Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO–gold nanoparticle–Nafion nanocomposite , 2009 .

[7]  Guo-Li Shen,et al.  Platinum nanoparticle-modified carbon fiber ultramicroelectrodes for mediator-free biosensing , 2006 .

[8]  Y. Wen,et al.  Direct electrochemistry and bioelectrocatalysis of horseradish peroxidase based on gold nano-seeds dotted TiO2 nanocomposite. , 2010, Biosensors & bioelectronics.

[9]  M. A. Sanromán,et al.  Polyelectrostatic immobilization of gold nanoparticles-modified peroxidase on alginate-coated gold electrode for mediatorless biosensor construction , 2009 .

[10]  Itamar Willner,et al.  Surface plasmon resonance analysis of antibiotics using imprinted boronic acid-functionalized Au nanoparticle composites. , 2010, Analytical chemistry.

[11]  N. C. Veitch,et al.  Horseradish peroxidase: a modern view of a classic enzyme. , 2004, Phytochemistry.

[12]  Linhai Zhuo,et al.  Direct electrochemistry of horseradish peroxidase immobilized on the layered calcium carbonate-gold nanoparticles inorganic hybrid composite. , 2010, Biosensors & bioelectronics.

[13]  J. Jiao,et al.  Using silver nanoparticle to enhance current response of biosensor. , 2005, Biosensors & bioelectronics.

[14]  P. Yáñez‐Sedeño,et al.  Gold nanoparticle-based electrochemical biosensors , 2005, Analytical and bioanalytical chemistry.

[15]  Julio Caballero,et al.  Supramolecular assembly of β-cyclodextrin-modified gold nanoparticles and Cu, Zn-superoxide dismutase on catalase , 2005 .

[16]  Junwei Di,et al.  Electrodeposition of gold nanoparticles on indium/tin oxide electrode for fabrication of a disposable hydrogen peroxide biosensor. , 2009, Talanta.

[17]  Y. Chai,et al.  Amperometric hydrogen peroxide biosensor based on the immobilization of HRP on DNA–silver nanohybrids and PDDA-protected gold nanoparticles , 2009 .

[18]  Michael Holzinger,et al.  Adamantane/beta-cyclodextrin affinity biosensors based on single-walled carbon nanotubes. , 2009, Biosensors & bioelectronics.

[19]  Jinxing Wang,et al.  Multilayered construction of glucose oxidase and gold nanoparticles on Au electrodes based on layer-by-layer covalent attachment , 2006 .

[20]  Xiaoling Yang,et al.  A novel hydrogen peroxide biosensor based on Au-graphene-HRP-chitosan biocomposites , 2010 .

[21]  M. Şenel,et al.  Amperometric hydrogen peroxide biosensor based on covalent immobilization of horseradish peroxidase on ferrocene containing polymeric mediator , 2010 .

[22]  J M Pingarrón,et al.  Development of a high analytical performance-tyrosinase biosensor based on a composite graphite-Teflon electrode modified with gold nanoparticles. , 2006, Biosensors & bioelectronics.

[23]  Serge Cosnier,et al.  Reagentless biosensor for hydrogen peroxide based on self-assembled films of horseradish peroxidase/laponite/chitosan and the primary investigation on the inhibitory effect by sulfide. , 2010, Biosensors & bioelectronics.

[24]  Y. Chai,et al.  Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles , 2010, Applied biochemistry and biotechnology.

[25]  A. Kaifer,et al.  Cyclodextrin-Modified Gold Nanospheres , 2000 .

[26]  S. Lim,et al.  A glucose biosensor based on electrodeposition of palladium nanoparticles and glucose oxidase onto Nafion-solubilized carbon nanotube electrode. , 2005, Biosensors & bioelectronics.

[27]  Xianfu Lin,et al.  One-step construction of reagentless biosensor based on chitosan-carbon nanotubes-nile blue-horseradish peroxidase biocomposite formed by electrodeposition. , 2009, Talanta.

[28]  X. Zhang,et al.  Facile Synthesis of Mercaptophenylboronic Acid-Functionalized Core−Shell Structure Fe3O4@C@Au Magnetic Microspheres for Selective Enrichment of Glycopeptides and Glycoproteins , 2010 .

[29]  Ashutosh Chilkoti,et al.  A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. , 2002, Analytical chemistry.

[30]  Minghui Yang,et al.  Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. , 2006, Biomaterials.

[31]  Aiguo Wu,et al.  A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network. , 2002, Analytical chemistry.

[32]  Y. Wen,et al.  Electrochemistry and electrocatalytic properties of mixed assemblies of horseradish peroxidase, poly(diallyl dimethylammonium chloride) and gold nanoparticles on a glassy carbon electrode , 2009 .

[33]  M. L. Mena,et al.  Bienzyme amperometric biosensor using gold nanoparticle-modified electrodes for the determination of inulin in foods. , 2008, Analytical biochemistry.

[34]  Li Wang,et al.  A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized on colloidal Au modified ITO electrode , 2004 .