Efeito do uso de óleo na dieta sobre a lipogênese e o perfil lipídico de tilápias-do-nilo

A total of 35 males of Nile tilapia, averaging initial weight of 205 g, were maintained in five 250 L metabolism boxes to evaluate the lipid metabolism of Nile tilapias (Oreochromis niloticus). The experiment lasted 30 days, from October to November 2006. The treatments were: control diet plus olive oil; control diet plus corn oil; control diet plus soybean oil; control diet plus linseed oil; control diet plus fish oil. The experiment was in a completely randomized design with five treatments and seven replicates. The evaluated parameters were: fatty acid profile of the muscle tissue and hepatic activity of glucose-6-phosphate dehydrogenase (G6PD) and malic enzyme (ME). The data were submitted to variance analysis and the means of the treatments were compared by Scott-Knott's test (5% of significance). The fish fed on a diet rich in polyunsaturated fatty acids presented increased contents of such fat acids in their lipid muscle composition. The hepatic activity of G6PD was superior to that of ME, being higher in the specimen fed rations containing olive, corn and soybean oil, thus, evidencing the highest lipid muscle deposition of these fish.

[1]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[2]  M. Sanden,et al.  Modulation of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malic enzyme activity by glucose and alanine in Atlantic salmon, Salmo salar L. hepatocytes , 2003 .

[3]  S. Panserat,et al.  Effect of dietary carbohydrate‐to‐lipid ratios on growth, lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis, Kaup) , 2004 .

[4]  Xuebing Xu,et al.  Effect of structured lipids based on fish oil on the growth and fatty acid composition in rainbow trout (Oncorhynchus mykiss) , 2005 .

[5]  A. von Schaewen,et al.  Purification, characterization, and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.). , 1994, The Plant journal : for cell and molecular biology.

[6]  M. Matsushita,et al.  The influence of feed supply time on the fatty acid profile of Nile tilapia (Oreochromis niloticus) fed on a diet enriched with n-3 fatty acids , 2003 .

[7]  J. G. Salway,et al.  Metabolism at a glance , 1993 .

[8]  J. Girard,et al.  Effect of diets rich in medium-chain and long-chain triglycerides on lipogenic-enzyme gene expression in liver and adipose tissue of the weaned rat. , 1992, European journal of biochemistry.

[9]  I. Metón,et al.  Short- and long-term effects of refeeding on key enzyme activities in glycolysis–gluconeogenesis in the liver of gilthead seabream (Sparus aurata) , 2003 .

[10]  C. López-Bote,et al.  Short-term modulation of lipogenesis by macronutrients in rainbow trout (Oncorhynchus mykiss) hepatocytes , 2000, British Journal of Nutrition.

[11]  A. Obach,et al.  Growth, digestibility and fatty acid utilization in large Atlantic salmon (Salmo salar) fed varying levels of n-3 and saturated fatty acids , 2003 .

[12]  Harold J. Bright,et al.  Purification and properties of L-malic enzyme from Escherichia coli , 1970 .

[13]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[14]  M. Matsushita,et al.  Fatty Acids Profile and Cholesterol Contents of Three Brazilian Brycon Freshwater Fishes , 2001 .

[15]  S. Kaushik,et al.  Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass (Dicentrarchus labrax) , 1998 .

[16]  G. Sampaio,et al.  The influence of season on the lipid profiles of five commercially important species of Brazilian fish , 2003 .

[17]  D. Tocher,et al.  The lipid composition and biochemistry of freshwater fish. , 1987, Progress in lipid research.

[18]  L. Morgan,et al.  Effects of dietary fatty acid composition on basal and hormone-stimulated hepatic lipogenesis and on circulating lipids in the rat , 1995, British Journal of Nutrition.

[19]  S. Kaushik,et al.  Dietary lipid level, hepatic lipogenesis and flesh quality in turbot (Psetta maxima) , 2001 .

[20]  M. Sánchez-Muros,et al.  Long‐term nutritional effects on the primary liver and kidney metabolism in rainbow trout (Oncorhynchus mykiss). II. Adaptive response of glucose 6‐phosphate dehydrogenase activity to high‐carbohydrate/low‐protein and high‐fat/non‐carbohydrate diets , 1996 .

[21]  D. Tocher Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish , 2003 .

[22]  N. Bandarra,et al.  Effects of dietary conjugated linoleic acid on growth, nutrient utilization, body composition, and hepatic lipogenesis in rainbow trout juveniles (Oncorhynchus mykiss) , 2005 .

[23]  Carmino Hayashi,et al.  Exigencia de proteina para alevino revertido de tilapia do Nilo (Oreochromis niloticus) , 2000 .

[24]  K. Mai,et al.  Effect of dietary lipid level on growth performance, lipid deposition, hepatic lipogenesis in juvenile cobia (Rachycentron canadum) , 2005 .

[25]  J. Bautista,et al.  Growth, lipogenesis and body composition of piracanjuba (Brycon orbignyanus) fingerlings fed different dietary protein and lipid concentrations , 2003 .

[26]  M. Matsushita,et al.  θ3 fatty acids in freshwater fish from south brazil , 1995 .