Determination of the exposure parameters that maximise the concentrations of the anaesthetic/sedative eugenol in rainbow trout (Oncorhynchus mykiss) skin-on fillet tissue

Studies were conducted to determine the anaesthetic/sedative concentrations and durations that would maximise anaesthetic/sedative residue concentrations in rainbow trout (Oncorhynchus mykiss) skin-on fillet tissue. Rainbow trout (167–404 g) were exposed to 50 mg l−1 AQUI-S® 20E (10% active ingredient, eugenol) in 17°C freshwater for durations up to 1440 min, 100 and 250 mg l−1 AQUI-S® 20E for durations up to 240 min, and 500 and 1000 mg l−1 AQUI-S® 20E for durations up to 90 min. Fish exposed to 100 mg l−1 AQUI-S® 20E for durations of 30, 60, 120 and 240 min had the greatest eugenol concentrations in the fillet tissue, 50, 58, 54 and 62 µg g−1, respectively. All other exposure concentrations and durations resulted in significantly lower eugenol concentrations, i.e. all < 39 µg g−1.

[1]  C. W. Luhning.  Residues of MS-222, benzocaine, and their metabolites in striped bass following anesthesia , 1973 .

[2]  H. H. Ku,et al.  Contributions to Probability and Statistics, Essays in Honor of Harold Hotelling. , 1961 .

[3]  R. A. Schoettger,et al.  Residues of MS-222 in four salmonids following anesthesia , 1967 .

[4]  J. Neter,et al.  Applied linear statistical models : regression, analysis of variance, and experimental designs , 1974 .

[5]  J. Meinertz,et al.  Evaluation of analytical techniques to determine AQUI-S® 20E (eugenol) concentrations in water , 2014 .

[6]  W. Hayton,et al.  Disposition of benzocaine in channel catfish , 1996 .

[7]  T. M. Schreier,et al.  Depletion of isoeugenol residues from the fillet tissue of AQUI-S™ exposed rainbow trout ( Oncorhynchus mykiss ) , 2009 .

[8]  T. M. Schreier,et al.  Isoeugenol concentrations in rainbow trout (Oncorhynchus mykiss) skin-on fillet tissue after exposure to AQUI-S™ at different temperatures, durations, and concentrations , 2006 .

[9]  G. R. Stehly,et al.  Metabolism, Elimination, and Pharmacokinetics of the Fish Anesthetic Benzocaine , 1999 .

[10]  G. R. Stehly,et al.  Effects of temperature on the elimination of benzocaine and acetylated benzocaine residues from the edible fillet of rainbow trout (Oncorhynchus mykiss) , 2000, Food additives and contaminants.

[11]  C. W. Luhning.  Methods for simultaneous determination and identification of MS-222 and metabolites in fish tissues , 1973 .

[12]  Robert Kearney,et al.  Accumulation and clearance of the anaesthetics clove oil and AQUI-S™ from the edible tissue of silver perch (Bidyanus bidyanus) , 2004 .

[13]  W. J. Langford.  Statistical Methods , 1959, Nature.

[14]  J. L. Allen.  Residues of Benzocaine in Rainbow Trout, Largemouth Bass, and Fish Meal , 1988 .

[15]  Clyde Young Kramer,et al.  Extension of multiple range tests to group means with unequal numbers of replications , 1956 .

[16]  P. Building.  BUREAU OF SPORT FISHERIES AND WILDLIFE , 2007 .

[17]  S. Shapiro,et al.  An Analysis of Variance Test for Normality (Complete Samples) , 1965 .

[18]  荒井保明.  Pharmacokinetics , 1993 .