Sequential immunohistochemistry: a promising new tool for the pathology laboratory

Current immunohistochemical methods to study the expression of multiple proteins in a single tissue section suffer from several limitations. In this article, we report on sequential immunohistochemistry (S‐IHC), a novel, easy method that allows the study of numerous proteins in a single tissue section, while requiring very limited optimization.

[1]  R. Siebert,et al.  FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue. , 2006, The Journal of molecular diagnostics : JMD.

[2]  Samir Kumar-Singh,et al.  Antibody Elution Method for Multiple Immunohistochemistry on Primary Antibodies Raised in the Same Species and of the Same Subtype , 2009, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[3]  Jason A Papin,et al.  Simple: A Sequential Immunoperoxidase Labeling and Erasing Method , 2009, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  Xianjin Zhou,et al.  System-Wide Immunohistochemical Analysis of Protein Co-Localization , 2012, PloS one.

[5]  J. Leonardelli,et al.  An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.