Long-distance FRET analysis: a Monte Carlo simulation study.

A new method for extending the utilizable range of Förster resonance energy transfer (FRET) is proposed and tested by the Monte Carlo technique. The obtained results indicate that the efficiency of FRET can be significantly enhanced at a given distance if the energy transfer takes place toward multiple acceptors that are closely located on a macromolecule instead of a single acceptor molecule as it is currently used in FRET analysis. On the other hand, reasonable FRET efficiency can be obtained at significantly longer distances than in the case of a single acceptor. Randomly distributed and parallel orientated acceptor transition moments with respect to the transition moment of the donor molecule have been analyzed as two extreme cases. As expected, a parallel orientation of donor and acceptor transition moments results in a more efficient excitation energy transfer. This finding could be used to directly reveal the assembly/deassembly of large protein complexes in a cell by fluorescence microscopy.

[1]  Michelle R. Arkin,et al.  Small-molecule inhibitors of protein–protein interactions: progressing towards the dream , 2004, Nature Reviews Drug Discovery.

[2]  P. Bojarski,et al.  Excitation energy transport between the ionic forms of rhodamine B in viscous solutions , 1998 .

[3]  Zygmunt Gryczynski,et al.  Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces. , 2004, Analytical biochemistry.

[4]  P. Bojarski,et al.  Reversible energy transfer between monomers and fluorescent dimers of rhodamine S in polyvinyl alcohol films , 2011 .

[5]  L. Kułak.  Hybrid Monte-Carlo simulations of fluorescence anisotropy decay in disordered two-component systems in the presence of forward and back energy transfer , 2008 .

[6]  Th. Förster.  Zwischenmolekulare Energiewanderung und Fluoreszenz , 1948 .

[7]  L. Stryer,et al.  Energy transfer: a spectroscopic ruler. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[8]  P. Bojarski,et al.  Unusual fluorescence anisotropy spectra of three-component donor–mediator–acceptor systems in uniaxially stretched polymer films in the presence of energy transfer , 2008 .

[9]  Joseph R. Lakowicz,et al.  Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA , 2004, Journal of Fluorescence.

[10]  B. Bagchi,et al.  Distance and orientation dependence of excitation energy transfer: from molecular systems to metal nanoparticles. , 2009, The journal of physical chemistry. B.

[11]  Paul S. Blank,et al.  Anomalous Surplus Energy Transfer Observed with Multiple FRET Acceptors , 2009, PloS one.

[12]  P. Bojarski,et al.  Nonradiative long range energy transfer in donor-acceptor systems with excluded volume , 2008 .

[13]  I. Gryczynski,et al.  Flavin mononucleotide fluorescence intensity decay in concentrated aqueous solutions , 2007 .

[14]  C. Schultz,et al.  Analysis of protein complex hierarchy in living cells. , 2008, ACS chemical biology.

[15]  S. Hell.  Far-Field Optical Nanoscopy , 2007, Science.

[16]  U. Krull,et al.  New opportunities in multiplexed optical bioanalyses using quantum dots and donor–acceptor interactions , 2010, Analytical and bioanalytical chemistry.

[17]  S. d'Auria,et al.  Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes. , 2009, ACS applied materials & interfaces.

[18]  Graham R Fleming,et al.  Dynamics of light harvesting in photosynthesis. , 2009, Annual review of physical chemistry.