Paper-based electrochemical sensing platform with integral battery and electrochromic read-out.

We report a battery-powered, microelectrochemical sensing platform that reports its output using an electrochromic display. The platform is fabricated based on paper fluidics and uses a Prussian blue spot electrodeposited on an indium-doped tin oxide thin film as the electrochromic indicator. The integrated metal/air battery powers both the electrochemical sensor and the electrochromic read-out, which are in electrical contact via a paper reservoir. The sample activates the battery and the presence of analyte in the sample initiates the color change of the Prussian blue spot. The entire system is assembled on the lab bench, without the need for cleanroom facilities. The applicability of the device to point-of-care sensing is demonstrated by qualitative detection of 0.1 mM glucose and H(2)O(2) in artificial urine samples.

[1]  David E. Williams,et al.  Point of care diagnostics: status and future. , 2012, Analytical chemistry.

[2]  R. Crooks,et al.  Self-powered sensor for naked-eye detection of serum trypsin. , 2011, Analytical chemistry.

[3]  George M Whitesides,et al.  Integration of paper-based microfluidic devices with commercial electrochemical readers. , 2010, Lab on a chip.

[4]  Shelley D Minteer,et al.  Nitroaromatic actuation of mitochondrial bioelectrocatalysis for self-powered explosive sensors. , 2008, Journal of the American Chemical Society.

[5]  G. Whitesides,et al.  Three-dimensional microfluidic devices fabricated in layered paper and tape , 2008, Proceedings of the National Academy of Sciences.

[6]  George M Whitesides,et al.  Electrochemical sensing in paper-based microfluidic devices. , 2010, Lab on a chip.

[7]  G. Whitesides,et al.  Diagnostics for the developing world: microfluidic paper-based analytical devices. , 2010, Analytical chemistry.

[8]  Yi Cui,et al.  Highly conductive paper for energy-storage devices , 2009, Proceedings of the National Academy of Sciences.

[9]  Joseph Wang,et al.  Portable electrochemical systems , 2002 .

[10]  I. Willner,et al.  Self-powered enzyme-based biosensors. , 2001, Journal of the American Chemical Society.

[11]  Orawon Chailapakul,et al.  Electrochemical detection for paper-based microfluidics. , 2009, Analytical chemistry.

[12]  Yi Lu,et al.  Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. , 2011, Nature chemistry.

[13]  Ki Bang Lee,et al.  Two-step activation of paper batteries for high power generation: design and fabrication of biofluid- and water-activated paper batteries , 2006 .

[14]  Kingo Itaya,et al.  Spectroelectrochemistry and electrochemical preparation method of Prussian blue modified electrodes , 1982 .

[15]  Ki Bang Lee,et al.  Urine-activated paper batteries for biosystems , 2005 .

[16]  M. Hilder,et al.  Paper-based, printed zinc–air battery , 2009 .