Microbial gutta percha degradation shares common steps with rubber 1 degradation by Nocardia nova SH 22 a 2 3

3 Quan Luo, Sebastian Hiessl, Anja Poehlein and Alexander Steinbüchel 4 5 Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität 6 Münster, Corrensstraße 3, 48149 Münster, Germany 7 Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institut für 8 Mikrobiologie und Genetik, Georg-August-Universität Göttingen, 37077 Göttingen, Germany 9 King Abdulaziz University, Faculty of Biology, Jeddah, Saudi Arabia 10 11 12 13 14 15 Correspondence: Alexander Steinbüchel, Institut für Molekulare Mikrobiologie und Biotechnologie, 16 Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany. Email: 17 steinbu@uni-muenster.de. Tel.: +49-251-8339821. Fax: +49-251-8338388. 18 19 20 21 Abbreviations: Amp, ampicillin; Apra, apramycin; BHI, brain heart infusion; CFU, colony forming units; 22 GP, gutta percha; INH, isonicotinic acid hydrazide; Km, kanamycin; MSM, mineral salts medium; NB, 23 nutrient broth; Neo, neomycin; NR, natural rubber; ORF, open reading frame; Thio, thiostrepton. 24 25 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.03016-12 AEM Accepts, published online ahead of print on 7 December 2012

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