Detection of Ilheus virus in mosquitoes from southeast Amazon, Brazil.

BACKGROUND Arbovirus surveillance in field-collected mosquitoes is essential in monitoring virus activity to avoid emergence and outbreaks of arboviruses. METHODS We used reverse transcription polymerase chain reaction methods to search for arbovirus in mosquitoes collected in Brazil's southeast Amazon forest remnants during 2015-2016. RESULTS We detected Iheus virus (ILHV) RNA in Culex declarator, Culex (Melanoconion) and Ochlerotatus serratus mosquitoes. CONCLUSIONS These results indicate ILHV circulation in the studied area, highlighting its potential emergence in human population. More studies are necessary to confirm the roles of these three species in ILHV maintenance.