Comparison of two fabricated aptasensors based on modified carbon paste/oleic acid and magnetic bar carbon paste/Fe3O4@oleic acid nanoparticle electrodes for tetracycline detection.

In this research, we have improved two aptasensors based on a modified carbon paste electrode (CPE) with oleic acid (OA), and a magnetic bar carbon paste electrode (MBCPE) with Fe3O4 magnetic nanoparticles and oleic acid (OA). After the immobilization process of anti-TET at the electrode surfaces, the aptasensors were named CPE/OA/anti-TET and MBCPE/Fe3O4NPs/OA/anti-TET respectively. In this paper, the detection of tetracycline is compared using CPE/OA/anti-TET and MBCPE/Fe3O4NPs/OA/anti-TET aptasensors. These modified electrodes were characterized by infrared spectroscopy (IR), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), UV-vis spectroscopy, and voltammetric methods. The linear range and the detection limit for TET with the CPE/OA/anti-TET aptasensor were found to be 1.0×10(-12)-1.0×10(-7)M and 3.0×10(-13)M respectively by EIS method. The linear range and the detection limit for TET with the CPE/OA/anti-TET aptasensor were found to be 1.0×10(-10)-1.0×10(-7)M with a limit of detection of 2.9×10(-11)M using differential pulse voltammetry (DPV) technique. The MBCPE/Fe3O4NPs/OA/anti-TET aptasensor was used for determination of TET, and a liner range of 1.0×10(-14)-1.0×10(-6)M with a detection limit of 3.8×10(-15)M was obtained by EIS method. Also, the linear range and detection limit of 1.0×10(-12)-1.0×10(-6)M and 3.1×10(-13)M respectively, were obtained for MBCPE/Fe3O4NPs/OA/anti-TET aptasensor using DPV. The proposed aptasensors were applied for determination of tetracycline in some real samples such as drug, milk, honey and blood serum samples.

[1]  Xu Wang,et al.  Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles , 2014, Sensors.

[2]  Joseph Wang,et al.  Magnetic-field stimulated DNA oxidation , 2002 .

[3]  Junyong Sun,et al.  Simple and novel electrochemical sensor for the determination of tetracycline based on iron/zinc cations-exchanged montmorillonite catalyst. , 2014, Talanta.

[4]  M. Gholivand,et al.  Electrooxidation behavior of warfarin in Fe3O4 nanoparticles modified carbon paste electrode and its determination in real samples. , 2015, Materials science & engineering. C, Materials for biological applications.

[5]  M. Gholivand,et al.  Electrochemical characterization of some bisphosphoramidates spiked carbon paste electrodes and their applications in DNA sensing , 2015 .

[6]  M. Helaleh,et al.  Field method for the micro-quantitative determination of tetracycline in human blood serum. , 1998, Journal of pharmaceutical and biomedical analysis.

[7]  A. Srivastava,et al.  Simultaneous electro-catalytic oxidative determination of ascorbic acid and folic acid using Fe3O4 nanoparticles modified carbon paste electrode , 2015 .

[8]  Ashok Mulchandani,et al.  Simple and label-free electrochemical impedance Amelogenin gene hybridization biosensing based on reduced graphene oxide. , 2014, Biosensors & bioelectronics.

[9]  N. E. Bari,et al.  Development of a novel sensitive molecularly imprinted polymer sensor based on electropolymerization of a microporous-metal-organic framework for tetracycline detection in honey , 2016 .

[10]  Development of a novel MWCNTs-triazene-modified carbon paste electrode for potentiometric assessment of Hg(II) in the aquatic environments. , 2015, Materials science & engineering. C, Materials for biological applications.

[11]  Chen Dan,et al.  Development of an aptasensor for electrochemical detection of tetracycline , 2013 .

[12]  J. Justin Gooding,et al.  Voltammetric determination of DNA hybridization using methylene blue and self-assembled alkanethiol monolayer on gold electrodes , 2002 .

[13]  Chunyan Sun,et al.  A novel colorimetric aptasensor using cysteamine-stabilized gold nanoparticles as probe for rapid and specific detection of tetracycline in raw milk , 2015 .

[14]  Dujuan Li,et al.  Electrochemical aptasensor for the detection of tetracycline with multi-walled carbon nanotubes amplification , 2012 .

[15]  Hao Zhou,et al.  Label-Free Electrochemical Detection of Tetracycline by an Aptamer Nano-Biosensor , 2012 .

[16]  Dianping Tang,et al.  Platinum-catalyzed hydrogen evolution reaction for sensitive electrochemical immunoassay of tetracycline residues , 2013 .

[17]  M. Stobiecka,et al.  Piezometric biosensors for anti-apoptotic protein survivin based on buried positive-potential barrier and immobilized monoclonal antibodies. , 2016, Biosensors & bioelectronics.

[18]  Wei Sun,et al.  Application of Fe3O4 mesoporous sphere modified carbon ionic liquid electrode as electrochemical hemoglobin biosensor. , 2013, Colloids and surfaces. B, Biointerfaces.

[19]  Z. Zhang,et al.  Flow-injection chemiluminescence determination of tetracyclines with in situ electrogenerated bromine as the oxidant , 2001 .

[20]  Su Jin Lee,et al.  Single-stranded DNA aptamers specific for antibiotics tetracyclines. , 2008, Bioorganic & medicinal chemistry.

[21]  Susana Campuzano,et al.  Integrated disposable electrochemical immunosensors for the simultaneous determination of sulfonamide and tetracycline antibiotics residues in milk. , 2013, Biosensors & bioelectronics.

[22]  Yiyang Dong,et al.  Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey. , 2014, Biosensors & bioelectronics.

[23]  Á. Maquieira,et al.  Synthesis of haptens and development of a sensitive immunoassay for tetracycline residues. Application to honey samples. , 2007, Analytica chimica acta.

[24]  Chao Liu,et al.  Synthesis of bilayer oleic acid-coated Fe3O4 nanoparticles and their application in pH-responsive Pickering emulsions. , 2007, Journal of colloid and interface science.

[25]  Xiao-li Xu,et al.  Development of an electrochemical aptamer-based sensor with a sensitive Fe3O4 nanopaticle-redox tag for reagentless protein detection , 2011 .

[26]  Xuefeng Guo,et al.  Exclusively selective oxidation of toluene to benzaldehyde on ceria nanocubes by molecular oxygen. , 2010, Chemical communications.

[27]  Yunlei Zhou,et al.  Electrochemical behaviour of Sudan I at Fe3O4 nanoparticles modified glassy carbon electrode and its determination in food samples. , 2011, Food chemistry.

[28]  B. Paull,et al.  Electrodeposition of palladium nanoparticles on porous graphitized carbon monolith modified carbon paste electrode for simultaneous enhanced determination of ascorbic acid and uric acid , 2015 .

[29]  Marilyn Roberts,et al.  Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance , 2001, Microbiology and Molecular Biology Reviews.

[30]  J. Miranda,et al.  Monitoring the presence of residues of tetracyclines in baby food samples by HPLC-MS/MS , 2014 .

[31]  Huabei Peng,et al.  Re-examination of characteristic FTIR spectrum of secondary layer in bilayer oleic acid-coated Fe3O4 nanoparticles , 2010 .

[32]  J. Miller,et al.  Statistics for Analytical Chemistry , 1993 .

[33]  A. Salimi,et al.  Highly sensitive electrochemical aptasensor for immunoglobulin E detection based on sandwich assay using enzyme-linked aptamer. , 2014, Analytical biochemistry.

[34]  Hong Yang,et al.  “Pulling” Nanoparticles into Water: Phase Transfer of Oleic Acid Stabilized Monodisperse Nanoparticles into Aqueous Solutions of α-Cyclodextrin , 2003 .

[35]  M. Ramezani,et al.  A novel colorimetric triple-helix molecular switch aptasensor for ultrasensitive detection of tetracycline. , 2015, Biosensors & bioelectronics.

[36]  V. Jovanovski,et al.  Silver particle-decorated carbon paste electrode based on ionic liquid for improved determination of nitrite , 2015 .

[37]  X. Xia,et al.  Characterization and application of Fe3O4/SiO2 nanocomposites , 2006 .

[38]  M. Mazloum‐Ardakani,et al.  A highly sensitive and selective electrochemical DNA biosensor to diagnose breast cancer , 2015 .

[39]  Marek Trojanowicz,et al.  Determination of pesticides using electrochemical biosensors , 1996 .

[40]  M. Mazloum‐Ardakani,et al.  Sex determination based on amelogenin DNA by modified electrode with gold nanoparticle. , 2013, Analytical biochemistry.

[41]  M. Hepel,et al.  Effect of buried potential barrier in label-less electrochemical immunodetection of glutathione and glutathione-capped gold nanoparticles. , 2011, Biosensors & bioelectronics.

[42]  J. Tashkhourian,et al.  A sensitive electrochemical sensor for determination of gallic acid based on SiO2 nanoparticle modified carbon paste electrode. , 2015, Materials science & engineering. C, Materials for biological applications.

[43]  Biyang Deng,et al.  Pharmacokinetics and residues of tetracycline in crucian carp muscle using capillary electrophoresis on-line coupled with electrochemiluminescence detection. , 2012, Food chemistry.

[44]  M. Aldissi,et al.  Challenges of electrochemical impedance spectroscopy in protein biosensing. , 2009, Analytical chemistry.

[45]  Haoqing Hou,et al.  Architecture of Fe3O4–graphene oxide nanocomposite and its application as a platform for amino acid biosensing , 2012 .

[46]  Jonathan M. Slater,et al.  The determination of tetracycline residues in food using a disposable screen-printed gold electrode (SPGE) , 2007 .