High throughput quality and safety assessment of packaged green produce using two optical oxygen sensor based systems

Abstract Two new analytical techniques, the GreenLight™ system for rapid enumeration of total viable counts (TVC) in food homogenates and the Optech™ system for non-destructive sensing of residual O 2 in package headspace were applied to examine a total of 206 packages of green produce with the aim to assess their main quality and safety characteristics. Three types of ready-to-eat salads—Iceberg lettuce, Caesar salad & Italian leaf mix salad—packed under different modified atmosphere (5% CO 2 , 5–60% O 2 ) and stored at 4 °C for up to 10 days were examined by monitoring their TVC and headspace O 2 , CO 2 . The new detection methodology was compared with traditional methods showing good correlation with Dansensor gas analyzer for O 2 ( R 2  = 0.9912) and with agar plating ISO4833:2003 method for TVC ( R 2  = 0.7749). The lowest TVC was achieved in 5% oxygen packed salad while in the other conditions showed no clear improvement. Both systems provide affordable high speed, sample throughput and proved themselves to be useful, convenient and capable of supplying important information about the key physical and biological parameters of individual packs.

[1]  Dmitri B. Papkovsky,et al.  Nondestructive Monitoring of Oxygen Profiles in Packaged Foods Using Phase-Fluorimetric Oxygen Sensor , 2001 .

[2]  A. E. Watada,et al.  Quality of fresh-cut produce , 1999 .

[3]  R. Green,et al.  Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay , 2009 .

[4]  A. Amanatidou,et al.  Effect of elevated oxygen and carbon dioxide on the surface growth of vegetable‐associated micro‐organisms , 1999, Journal of applied microbiology.

[5]  F Devlieghere,et al.  Effect of high oxygen modified atmosphere packaging on microbial growth and sensorial qualities of fresh-cut produce. , 2001, International journal of food microbiology.

[6]  D. G. Mercer,et al.  Influence of a commercial warm chlorinated water treatment and packaging on the shelf-life of ready-to-use lettuce , 2004 .

[7]  Ana Allende,et al.  Microbial and quality changes in minimally processed baby spinach leaves stored under super atmospheric oxygen and modified atmosphere conditions , 2004 .

[8]  H. Scheer,et al.  A Red-Shifted Chlorophyll , 2010, Science.

[9]  Dmitri B. Papkovsky,et al.  Rapid High-Throughput Assessment of Aerobic Bacteria in Complex Samples by Fluorescence-Based Oxygen Respirometry , 2006, Applied and Environmental Microbiology.

[10]  Frank Devlieghere,et al.  Combining high oxygen atmospheres with low oxygen modified atmosphere packaging to improve the keeping quality of strawberries and raspberries , 2002 .

[11]  Gemma Oms-Oliu,et al.  The use of packaging techniques to maintain freshness in fresh-cut fruits and vegetables: a review , 2009 .

[12]  Dermot Diamond,et al.  Development of a volatile amine sensor for the monitoring of fish spoilage. , 2006, Talanta.

[13]  Dmitri B. Papkovsky,et al.  Phosphorescent Sensor Approach for Non-Destructive Measurement of Oxygen in Packaged Foods: Optimisation of Disposable Oxygen Sensors and their Characterization Over a Wide Temperature Range , 2000 .

[14]  L. Jacxsens,et al.  Effect of superatmospheric oxygen packaging on sensorial quality, spoilage, and Listeria monocytogenes and Aeromonas caviae growth in fresh processed mixed salads. , 2002, Journal of food protection.

[15]  R. Hayes,et al.  Genetic Variation for Shelf-life of Salad-cut Lettuce in Modified-atmosphere Environments , 2008 .

[16]  Milena Sinigaglia,et al.  Use of biodegradable films for prolonging the shelf life of minimally processed lettuce , 2008 .

[17]  J. Kerry,et al.  Physical assessment of composite biodegradable films manufactured using whey protein isolate, gelatin and sodium alginate. , 2010 .

[18]  Yang Tao,et al.  Effect of initial oxygen concentration and film oxygen transmission rate on the quality of fresh-cut romaine lettuce , 2005 .

[19]  Colette McDonagh,et al.  Sol-gel based optical carbon dioxide sensor employing dual luminophore referencing for application in food packaging technology. , 2002, The Analyst.

[20]  Sandhya. Modified atmosphere packaging of fresh produce: Current status and future needs , 2010 .

[21]  J. Kerry,et al.  Analysis of total aerobic viable counts in raw fish by high-throughput optical oxygen respirometry. , 2011, Journal of food protection.

[22]  Dmitri B. Papkovsky,et al.  Non-destructive assessment of oxygen levels in industrial modified atmosphere packaged cheddar cheese , 2006 .