Optical properties of an anterior lamellar human cornea model based on fibrin-agarose

The optical evaluation carried out using the Inverse Adding-Doubling (IAD) method to determine the scattering and the absorption coefficients of the bioengineered human corneal stromas showed that this type of artificial biomaterials shared many similarities with native control cornea after four weeks of development in culture. Their absorption and reduced scattering coefficients values were higher than the ones of the control cornea, but their spectral behaviors of both coefficients were similar. Time of development in culture was an influencing factor on the results.

[1]  T. J. T. P. Van Den Berg,et al.  Light transmittance of the human cornea from 320 to 700 nm for different ages , 1994, Vision Research.

[2]  C. Connon,et al.  Transparency, swelling and scarring in the corneal stroma , 2003, Eye.

[3]  A. N. Bashkatov,et al.  Optical properties of human skin, subcutaneous and mucous tissues in the wavelength range from 400 to 2000 nm , 2005 .

[4]  R. Ghinea,et al.  Investigating a novel nanostructured fibrin-agarose biomaterial for human cornea tissue engineering: rheological properties. , 2011, Journal of the mechanical behavior of biomedical materials.

[5]  Dhiraj K. Sardar,et al.  Optical absorption and scattering of bovine cornea, lens, and retina in the near-infrared region , 2012, Lasers in Medical Science.

[6]  M. Sefton,et al.  Tissue engineering. , 1998, Journal of cutaneous medicine and surgery.

[7]  Dhiraj K. Sardar,et al.  Optical absorption and scattering of bovine cornea, lens and retina in the visible region , 2009, Lasers in Medical Science.

[8]  F. Fankhauser,et al.  Applanation-free femtosecond laser processing of the cornea , 2011, Biomedical optics express.

[9]  Alexander Podskochy,et al.  Protective role of corneal epithelium against ultraviolet radiation damage. , 2004, Acta ophthalmologica Scandinavica.

[10]  A. Ionescu,et al.  Generation of bioengineered corneas with decellularized xenografts and human keratocytes. , 2011, Investigative ophthalmology & visual science.

[11]  Scott A Prahl,et al.  Preparation and characterization of polyurethane optical phantoms. , 2006, Journal of biomedical optics.

[12]  A. I. Schneider,et al.  Constructing an in vitro cornea from cultures of the three specific corneal cell types , 1999, In Vitro Cellular & Developmental Biology - Animal.

[13]  A. Welch,et al.  A review of the optical properties of biological tissues , 1990 .

[14]  Miguel Alaminos,et al.  Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold. , 2006, Investigative ophthalmology & visual science.

[15]  L Ventura,et al.  System for measuring the transmission spectrum of 'in vitro' corneas. , 2000, Physiological measurement.

[16]  Kohji Nishida,et al.  Tissue Engineering of the Cornea , 2003, Cornea.

[17]  B. Ambati,et al.  Corneal transparency: Genesis, maintenance and dysfunction , 2010, Brain Research Bulletin.

[18]  A. Welch,et al.  Determining the optical properties of turbid mediaby using the adding-doubling method. , 1993, Applied optics.

[19]  V. Tuchin.  Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis , 2000 .

[20]  Z. Cui,et al.  Mechanisms of structure generation during plastic compression of nanofibrillar collagen hydrogel scaffolds: towards engineering of collagen , 2011, Journal of tissue engineering and regenerative medicine.

[21]  Dhiraj K Sardar,et al.  Optical scattering, absorption, and polarization of healthy and neovascularized human retinal tissues. , 2005, Journal of biomedical optics.

[22]  T. Møller-Pedersen,et al.  The cellular basis of corneal transparency: evidence for 'corneal crystallins'. , 1999, Journal of cell science.

[23]  Edward W. Larsen,et al.  Light transport in biological tissue based on the simplified spherical harmonics equations , 2006, J. Comput. Phys..

[24]  M. M. Pérez,et al.  Transparency in a Fibrin and Fibrin–Agarose Corneal Stroma Substitute Generated by Tissue Engineering , 2011, Cornea.

[25]  Judith R. Mourant,et al.  Light scattering from cells: the contribution of the nucleus and the effects of proliferative status , 2000 .

[26]  Stephan Reichl,et al.  The use of a porcine organotypic cornea construct for permeation studies from formulations containing befunolol hydrochloride. , 2003, International journal of pharmaceutics.