Age and sex differences in human skeletal muscle: Role of reactive oxygen species

Previous studies, conducted on experimental animals, have indicated that reactive oxygen species (ROS) are involved in the aging process. The objective of this work was to study the relationship between oxidative damage and human skeletal muscle aging, measuring the activity of the main antioxidant enzymes superoxide dismutase (total and MnSOD), glutathione peroxidase (GPx) and catalase in the skeletal muscle of men and women in the age groups: young (17–40 years), adult (41–65 years) and aged (66–91 years). We also measured glutathione and glutathione disulfide (GSH and GSSG) levels and the redox index; lipid peroxidation and protein carbonyl content. Total SOD activity was lower in the 66–91 year-old vs. the 17–40 year-old men; MnSOD activity was significantly greater in 66–91 year-old vs. 17–40 year-old women. GPx activity remained unchanged. The activity of catalase was lower in adults than in young men but higher in the aged. We observed no changes in GSH levels and significantly higher GSSG levels only in aged men vs. adult men, and a significant decrease in aged women vs. aged men. The protein carbonyl content increased significantly in the 41–65 and 66–91 year-old vs. the 17–40 year-old men. Finally, young women have lower lipid peroxidation levels than young men. Significantly higher lipid peroxidation levels were observed in aged men vs. both young and adult men, and the same trend was noticed for women. We conclude that oxidative damage may play a crucial role in the decline of functional activity in human skeletal muscle with normal aging in both sexes; and that men appear to be more subject to oxidative stress than women.

[1]  O. Pansarasa,et al.  Age-dependent changes of antioxidant activities and markers of free radical damage in human skeletal muscle. , 1999, Free radical biology & medicine.

[2]  P. Mecocci,et al.  Age-dependent increases in oxidative damage to DNA, lipids, and proteins in human skeletal muscle. , 1999, Free radical biology & medicine.

[3]  M. Mattson,et al.  Estrogens Attenuate and Corticosterone Exacerbates Excitotoxicity, Oxidative Injury, and Amyloid β‐Peptide Toxicity in Hippocampal Neurons , 1996, Journal of neurochemistry.

[4]  S. Ameshima,et al.  [Glutathione peroxidase]. , 1995, Nihon rinsho. Japanese journal of clinical medicine.

[5]  B. Ames,et al.  Oxidative damage and mitochondrial decay in aging. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[6]  S. Papa,et al.  Decline with age of the respiratory chain activity in human skeletal muscle. , 1994, Biochimica et biophysica acta.

[7]  I. Inyang,et al.  Chloroquine reduces blood pressure and forearm vascular resistance and increases forearm blood flow in healthy young adults. , 1993, Clinical physiology.

[8]  D. Knook,et al.  Physiopathological Processes of Aging. Towards a Multicausal Interpretation. Proceedings of the 4th International Congress of the International Association of Biomedical Gerontology. Ancona, Italy, June 26-29, 1991. , 1992, Annals of the New York Academy of Sciences.

[9]  L. Ji,et al.  Glutathione and antioxidant enzymes in skeletal muscle: effects of fiber type and exercise intensity. , 1992, Journal of applied physiology.

[10]  B. Ames,et al.  Protein oxidation associated with aging is reduced by dietary restriction of protein or calories. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Carrillo,et al.  Age-related changes in antioxidant enzyme activities are region and organ, as well as sex, selective in the rat , 1992, Mechanisms of Ageing and Development.

[12]  M. Brown,et al.  Skeletal muscle adaptations to endurance training in 60- to 70-yr-old men and women. , 1992, Journal of applied physiology.

[13]  R. S. Sohal.  Hydrogen peroxide production by mitochondria may be a biomarker of aging , 1991, Mechanisms of Ageing and Development.

[14]  L. E. Rikans,et al.  Sex-dependent differences in the effects of aging on antioxidant defense mechanisms of rat liver. , 1991, Biochimica et biophysica acta.

[15]  L. Ji,et al.  Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. , 1990, The American journal of physiology.

[16]  E. Stadtman,et al.  Determination of carbonyl content in oxidatively modified proteins. , 1990, Methods in enzymology.

[17]  H. Esterbauer,et al.  Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. , 1990, Methods in enzymology.

[18]  B. Fanburg,et al.  Regulation of cellular glutathione. , 1989, The American journal of physiology.

[19]  E. Stadtman.  Protein modification in aging. , 1988, Journal of gerontology.

[20]  M. Anderson,et al.  Determination of glutathione and glutathione disulfide in biological samples. , 1985, Methods in enzymology.

[21]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[22]  P. Hegarty,et al.  Copper-zinc and manganese superoxide dismutase activities in cardiac and skeletal muscles during aging in male rats. , 1984, Gerontology.

[23]  L. Flohé,et al.  Superoxide dismutase assays. , 1984, Methods in enzymology.

[24]  L. Flohé,et al.  Assays of glutathione peroxidase. , 1984, Methods in enzymology.

[25]  B. Saltin,et al.  The ageing muscle. , 1983, Clinical physiology.

[26]  L. Larsson,et al.  Skeletal muscle metabolism and ultrastructure in relation to age in sedentary men. , 1978, Acta physiologica Scandinavica.

[27]  D. Harman.  Aging: a theory based on free radical and radiation chemistry. , 1956, Journal of gerontology.

[28]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.