Modulatory effect of quercetin on DNA damage, induced by etoposide in bone marrow cells and on changes in the activity of antioxidant enzymes in rats.

The aim of the studies was to evaluate the influence of quercetin on etoposide-induced DNA damage in rat bone marrow cells and on changes in the activities of superoxide dismutase (SOD), catalase and antioxidant power in lung tissue (AOP). Quercetin was administrated by gavage at a dose of 135 mg/kg b.w. for three consecutive days, and one hour after the last dose, etoposide was intraperitoneally injected, at dose of 80 mg/kg b.w. Bone marrow cells were harvested from the femurs 24 hours later and DNA damage was evaluated by the comet assay, under alkaline condition. The study provided evidence that quercetin, administered to rats, provides major protection against etoposide-induced DNA damage in bone marrow cells and does not influence either the SOD activity, lowered by etoposide, or AOP, enhanced by etoposide.

[1]  V. Breinholt,et al.  Effects of Commonly Consumed Fruit Juices and Carbohydrates on Redox Status and Anticancer Biomarkers in Female Rats , 2003, Nutrition and cancer.

[2]  T. Andoh.  [Mechanism of occurrence of secondary tumors by antitumor drugs]. , 1999, Gan to kagaku ryoho. Cancer & chemotherapy.

[3]  Y. Tyurina,et al.  Mechanism-based chemopreventive strategies against etoposide-induced acute myeloid leukemia: free radical/antioxidant approach. , 1999, Molecular pharmacology.

[4]  G. Kuttan,et al.  Myeloprotective effect of a non-toxic indigenous preparation Rasayana in cancer patients receiving chemotherapy and radiation therapy. A pilot study. , 1999, Journal of experimental & clinical cancer research : CR.

[5]  P. Hollman,et al.  Absorption, metabolism and health effects of dietary flavonoids in man. , 1997, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[6]  V. Mohsenin,et al.  Adaptation of lung antioxidants to cigarette smoking in humans. , 1996, Chest.

[7]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[8]  D. Roos,et al.  Protection of human neutrophils by endogenous catalase: studies with cells from catalase-deficient individuals. , 1980, The Journal of clinical investigation.

[9]  I. Fridovich,et al.  The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. , 1972, The Journal of biological chemistry.

[10]  R. Tice,et al.  The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. , 1991, Advances in experimental medicine and biology.

[11]  S. Phan,et al.  Oxygen metabolite detoxifying enzyme levels in bleomycin-induced fibrotic lungs. , 1988, Free radical biology & medicine.