Human polyomavirus 7-associated pruritic rash and viremia in transplant recipients.

Human polyomavirus 7 (HPyV7) is one of 11 HPyVs recently discovered through genomic sequencing technologies. Two lung transplant recipients receiving immunosuppressive therapy developed pruritic, brown plaques on the trunk and extremities showing a distinctive epidermal hyperplasia with virus-laden keratinocytes containing densely packed 36-45-nm icosahedral capsids. Rolling circle amplification and gradient centrifugation testing were positive for encapsidated HPyV7 DNA in skin and peripheral blood specimens from both patients, and HPyV7 early and capsid proteins were abundantly expressed in affected tissues. We describe for the first time that HPyV7 is associated with novel pathogenicity in some immunosuppressed individuals.

[1]  M. Feltkamp,et al.  Different Serologic Behavior of MCPyV, TSPyV, HPyV6, HPyV7 and HPyV9 Polyomaviruses Found on the Skin , 2013, PloS one.

[2]  I. Sauer,et al.  Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract , 2013, PloS one.

[3]  J. Decaprio,et al.  A cornucopia of human polyomaviruses , 2013, Nature Reviews Microbiology.

[4]  K. Khalili,et al.  The Rapidly Expanding Family of Human Polyomaviruses: Recent Developments in Understanding Their Life Cycle and Role in Human Pathology , 2013, PLoS pathogens.

[5]  O. Stine,et al.  Discovery of STL polyomavirus, a polyomavirus of ancestral recombinant origin that encodes a unique T antigen by alternative splicing. , 2013, Virology.

[6]  P. Coursaget,et al.  Age-Specific Seroprevalences of Merkel Cell Polyomavirus, Human Polyomaviruses 6, 7, and 9, and Trichodysplasia Spinulosa-Associated Polyomavirus , 2013, Clinical and Vaccine Immunology.

[7]  C. Buck,et al.  Characterization of monoclonal antibodies specific for the Merkel cell polyomavirus capsid. , 2010, Virology.

[8]  M. Feltkamp,et al.  Discovery of a New Human Polyomavirus Associated with Trichodysplasia Spinulosa in an Immunocompromized Patient , 2010, PLoS pathogens.

[9]  C. Buck,et al.  Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. , 2010, Cell host & microbe.

[10]  Yuan Chang,et al.  Merkel Cell Polyomavirus-Infected Merkel Cell Carcinoma Cells Require Expression of Viral T Antigens , 2010, Journal of Virology.

[11]  S. Swerdlow,et al.  Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors , 2009, International journal of cancer.

[12]  Yuan Chang,et al.  T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus , 2008, Proceedings of the National Academy of Sciences.

[13]  G. Giraud,et al.  DNA from BK Virus and JC Virus and from KI, WU, and MC Polyomaviruses as Well as from Simian Virus 40 Is Not Detected in Non-UV-Light-Associated Primary Malignant Melanomas of Mucous Membranes , 2008, Journal of Clinical Microbiology.

[14]  C. Buck,et al.  Production of Papillomavirus‐Based Gene Transfer Vectors , 2007, Current protocols in cell biology.

[15]  R. Johne,et al.  Novel Polyomavirus Detected in the Feces of a Chimpanzee by Nested Broad-Spectrum PCR , 2005, Journal of Virology.

[16]  X. Huang,et al.  CAP3: A DNA sequence assembly program. , 1999, Genome research.

[17]  E. Pettersen,et al.  Use of multiple PCR primer sets for optimal detection of human papillomavirus , 1996, Journal of clinical microbiology.

[18]  D. Pim,et al.  Monoclonal antibodies specific for simian virus 40 tumor antigens , 1981, Journal of virology.

[19]  B. Thiers,et al.  Clonal Integration of a Polyomavirus in Human Merkel Cell Carcinoma , 2009 .