Interface-specific x-ray phase retrieval tomography of complex biological organs

We demonstrate interface-specific propagation-based x-ray phase retrieval tomography of the thorax and brain of small animals. Our method utilizes a single propagation-based x-ray phase-contrast image per projection, under the assumptions of (i) partially coherent paraxial radiation, (ii) a static object whose refractive indices take on one of a series of distinct values at each point in space and (iii) the projection approximation. For the biological samples used here, there was a 9-200 fold improvement in the signal-to-noise ratio of the phase-retrieved tomograms over the conventional attenuation-contrast signal. The ability to 'digitally dissect' a biological specimen, using only a single phase-contrast image per projection, will be useful for low-dose high-spatial-resolution biomedical imaging of form and biological function in both healthy and diseased tissue.

[1]  S. Wilkins,et al.  Contrast and resolution in imaging with a microfocus x-ray source , 1997 .

[2]  S. Wilkins,et al.  Phase-contrast imaging using polychromatic hard X-rays , 1996, Nature.

[3]  Avinash C. Kak,et al.  Principles of computerized tomographic imaging , 2001, Classics in applied mathematics.

[4]  A Bravin,et al.  High-resolution brain tumor visualization using three-dimensional x-ray phase contrast tomography , 2007, Physics in medicine and biology.

[5]  S. Hooper,et al.  A new design for high stability pressure-controlled ventilation for small animal lung imaging , 2010 .

[6]  L. Pellegrino,et al.  stereotaxic atlas of the rat brain , 1967 .

[7]  S. Wilkins,et al.  X‐ray omni microscopy , 2004, Journal of microscopy.

[8]  T E Gureyev,et al.  Phase-contrast tomography of single-material objects from few projections. , 2008, Optics express.

[9]  K. Nugent,et al.  Noninterferometric phase imaging with partially coherent light , 1998 .

[10]  R.G. Baraniuk,et al.  Compressive Sensing [Lecture Notes] , 2007, IEEE Signal Processing Magazine.

[11]  M. Teich,et al.  Fundamentals of Photonics , 1991 .

[12]  D. Paterson,et al.  Quantitative X-ray phase tomography with sub-micron resolution , 2003 .

[13]  T E Gureyev,et al.  An extension of Quasi-One-Dimensional Tomography. , 1998, Applied optics.

[14]  M. Teague.  Deterministic phase retrieval: a Green’s function solution , 1983 .

[15]  T. Gureyev,et al.  The binary dissector: phase contrast tomography of two- and three-material objects from few projections. , 2008, Optics express.

[16]  R. Scholten,et al.  Off-resonant defocus-contrast imaging of cold atoms. , 2004, Optics letters.

[17]  S. Wilkins,et al.  Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object , 2002, Journal of microscopy.

[18]  Xiaochuan Pan,et al.  Image reconstruction exploiting object sparsity in boundary-enhanced X-ray phase-contrast tomography. , 2010, Optics express.

[19]  S. Wilkins,et al.  X-ray phase-contrast microscopy and microtomography. , 2003, Optics express.

[20]  Kentaro Uesugi,et al.  Imaging lung aeration and lung liquid clearance at birth , 2007, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[21]  E. Wolf.  Three-dimensional structure determination of semi-transparent objects from holographic data , 1969 .

[22]  D. Paganin,et al.  2D and 3D X-ray phase retrieval of multi-material objects using a single defocus distance. , 2010, Optics express.

[23]  K. Nugent,et al.  Rapid quantitative phase imaging using the transport of intensity equation , 1997 .

[24]  Anton Maksimenko,et al.  First experiments on the Australian Synchrotron Imaging and Medical beamline, including investigations of the effective source size in respect of X-ray imaging. , 2010, Journal of synchrotron radiation.

[25]  Françoise Peyrin,et al.  Observation of microstructure and damage in materials by phase sensitive radiography and tomography , 1997 .

[26]  Richard G. Baraniuk,et al.  Compressive Sensing , 2008, Computer Vision, A Reference Guide.

[27]  Xiaochuan Pan,et al.  Analytic image reconstruction in local phase-contrast tomography. , 2004, Physics in medicine and biology.

[28]  Nicky Eshtiaghi,et al.  Phase retrieval tomography in the presence of noise , 2010 .

[29]  D. Paganin,et al.  On the origin of speckle in x-ray phase contrast images of lung tissue. , 2004, Physics in medicine and biology.

[30]  Timur E. Gureyev,et al.  Polychromatic cone-beam phase-contrast tomography , 2007 .

[31]  K. Nugent,et al.  Phase retrieval with the transport-of-intensity equation. II. Orthogonal series solution for nonuniform illumination , 1996 .

[33]  Akira Furukawa,et al.  The First Trial of Phase Contrast Imaging for Digital Full-Field Mammography Using a Practical Molybdenum X-Ray Tube , 2005, Investigative radiology.

[34]  K. Umetani,et al.  Construction and commissioning of a 215-m-long beamline at SPring-8 , 2001 .

[35]  E. Förster,et al.  Double crystal diffractometry for the characterization of targets for laser fusion experiments , 1980 .

[36]  Atsushi Momose,et al.  Phase–contrast X–ray computed tomography for observing biological soft tissues , 1996, Nature Medicine.

[37]  T. Gureyev,et al.  Quantitative quasi-local tomography using absorption and phase contrast , 2007 .

[38]  P. Cloetens,et al.  Holotomography: Quantitative phase tomography with micrometer resolution using hard synchrotron radiation x rays , 1999 .

[39]  P. Cloetens,et al.  Mixed transfer function and transport of intensity approach for phase retrieval in the Fresnel region. , 2007, Optics letters.

[40]  Kentaro Uesugi,et al.  X-ray refraction-enhanced imaging and a method for phase retrieval for a simple object. , 2002, Journal of synchrotron radiation.

[41]  Keith A. Nugent,et al.  Phase contrast radiography. II. Imaging of complex objects , 2005 .

[42]  K K W Siu,et al.  Dynamic measures of regional lung air volume using phase contrast x-ray imaging , 2008, Physics in medicine and biology.

[43]  A. Bronnikov,et al.  Theory of quantitative phase-contrast computed tomography. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[44]  Timur E. Gureyev,et al.  A general few-projection method for tomographic reconstruction of samples consisting of several distinct materials , 2010 .

[45]  S. Wilkins,et al.  Phase-and-amplitude computer tomography , 2006 .

[46]  P. Cloetens,et al.  Phase objects in synchrotron radiation hard x-ray imaging , 1996 .

[47]  A. Snigirev,et al.  On the possibilities of x-ray phase contrast microimaging by coherent high-energy synchrotron radiation , 1995 .

[48]  Stuart R. Stock,et al.  Developments in X-Ray Tomography VII , 2010 .

[49]  Phase retrieval for improved three-dimensional velocimetry of dynamic x-ray blood speckle , 2008 .

[50]  M A Anastasio,et al.  Computationally efficient and statistically robust image reconstruction in three-dimensional diffraction tomography. , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.