Quantum dot based immunosensor using 3D circular microchannels fabricated in PDMS.

Microchannel is basic functional component of microfluidic chip and every step-forward of its construction technique has been receiving concern all over the world. The present work describes a novel, rapid and simple fabrication technique for building 3D microchannels in poly(dimethyl siloxane) (PDMS) elastomer. These microchannels were used for rapid detection of antigens (E. coli) by quantum dot (QD) based approach. Luminescent QD (CdTe) were synthesized by aqueous method and characterized using high resolution transmission electron microscopy (HRTEM), fluorescence spectroscopy and X-ray diffraction (XRD). The QDs were functionalized with anti-E. coli antibodies for immuno-detection. The reported process allowed easier and faster method of fabrication of circular 3D micochannels and demonstrated their potential use in an immuno-biosensor device.

[1]  H. Ache.  Chemische Aspekte der Fusionstechnologie , 1989 .

[2]  J. Donegan,et al.  Optimisation of the synthesis and modification of CdTe quantum dots for enhanced live cell imaging , 2006 .

[3]  Alan Waggoner,et al.  Fluorescent labels for proteomics and genomics. , 2006, Current opinion in chemical biology.

[4]  M. Roco.  Nanotechnology: convergence with modern biology and medicine. , 2003, Current opinion in biotechnology.

[5]  Tae Jin Kim,et al.  A rapid and simple fabrication method for 3-dimensional circular microfluidic channel using metal wire removal process , 2010 .

[6]  Kunbao Cai,et al.  PDMS microchannel fabrication technique based on microwire-molding , 2008 .

[7]  Dong-Pyo Kim,et al.  Facile single step fabrication of microchannels with varying size. , 2009, Lab on a chip.

[8]  Andrew J deMello,et al.  Miniaturization: Chemistry at the crossroads. , 2009, Nature chemistry.

[9]  Qingrong Huang,et al.  Antibody-Conjugated CdTe Quantum Dots for Escherichia coli Detection , 2008 .

[10]  Paras N. Prasad,et al.  Introduction to Biophotonics , 2003 .

[11]  A. Rogach,et al.  Synthesis and characterization of thiol-stabilized CdTe nanocrystals , 1996 .

[12]  G. Whitesides,et al.  Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies , 2003, Electrophoresis.

[13]  Kin Fong Lei,et al.  Microfluidic channel fabrication by PDMS-interface bonding , 2006 .

[14]  E. Goldys.  Fluorescence Applications in Biotechnology and Life Sciences , 2009 .

[15]  J. Friend,et al.  Fabrication of microfluidic devices using polydimethylsiloxane. , 2010, Biomicrofluidics.

[16]  Limu Wang,et al.  A simple method for fabricating multi-layer PDMS structures for 3D microfluidic chips. , 2010, Lab on a chip.