Use of principal component analysis for differentiation of gelatine sources based on polypeptide molecular weights.

The study was aimed to differentiate between porcine and bovine gelatines in adulterated samples by utilising sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) combined with principal component analysis (PCA). The distinct polypeptide patterns of 6 porcine type A and 6 bovine type B gelatines at molecular weight ranged from 50 to 220 kDa were studied. Experimental samples of raw gelatine were prepared by adding porcine gelatine in a proportion ranging from 5% to 50% (v/v) to bovine gelatine and vice versa. The method used was able to detect 5% porcine gelatine added to the bovine gelatine. There were no differences in the electrophoretic profiles of the jelly samples when the proteins were extracted with an acetone precipitation method. The simple approach employing SDS-PAGE and PCA reported in this paper may provide a useful tool for food authenticity issues concerning gelatine.

[1]  H. Mayer.  Milk species identification in cheese varieties using electrophoretic, chromatographic and PCR techniques , 2005 .

[2]  Angela Chambery,et al.  Peptide fingerprint of high quality Campania white wines by MALDI-TOF mass spectrometry , 2009 .

[3]  H. Abdollahi,et al.  Differentiation of bovine and porcine gelatins using principal component analysis. , 2004, Journal of pharmaceutical and biomedical analysis.

[4]  M. Mulholland,et al.  Forensic classification of ballpoint pen inks using high performance liquid chromatography and infrared spectroscopy with principal components analysis and linear discriminant analysis , 2006 .

[5]  Yuzhi Wang,et al.  Fingerprint profile of active components for Andrographis paniculata Nees by HPLC-DAD , 2009 .

[6]  A. M. Marina,et al.  Use of the SAW Sensor Electronic Nose for Detecting the Adulteration of Virgin Coconut Oil with RBD Palm Kernel Olein , 2010 .

[7]  F. Jessen,et al.  Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: a collaborative study , 2000 .

[8]  Dong-Sun Lee,et al.  Characterization of fatty acids composition in vegetable oils by gas chromatography and chemometrics , 1998 .

[9]  J. Muyonga,et al.  Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin , 2004 .

[10]  M. Montowska,et al.  Species-specific expression of various proteins in meat tissue: proteomic analysis of raw and cooked meat and meat products made from beef, pork and selected poultry species. , 2012, Food chemistry.

[11]  D. Levieux,et al.  Differentiation of Gelatins Using Polyclonal Antibodies Raised Against Tyrosylated Bovine and Porcine Gelatins , 2005, Journal of immunoassay & immunochemistry.

[12]  H. Cai,et al.  Real-time PCR assays for detection and quantitation of porcine and bovine DNA in gelatin mixtures and gelatin capsules , 2012 .

[13]  S. Kędracka-Krok,et al.  Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis , 2010, Electrophoresis.

[14]  P. D. Jolley,et al.  Investigation of methods to detect mechanically recovered meat in meat products - II: Gel electrophoresis. , 1995, Meat science.

[15]  Dzulkifly Mat Hashim,et al.  Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins , 2010 .

[16]  Hamide Z Senyuva,et al.  Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication. , 2012, Meat science.

[17]  Christophe Cordella,et al.  Recent developments in food characterization and adulteration detection: technique-oriented perspectives. , 2002, Journal of agricultural and food chemistry.

[18]  S. Tanabe,et al.  A reliable enzyme linked immunosorbent assay for the determination of bovine and porcine gelatin in processed foods. , 2009, Journal of agricultural and food chemistry.

[19]  D. Levieux,et al.  Differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect ELISA. , 2005, Journal of pharmaceutical and biomedical analysis.

[20]  D. Kara,et al.  Evaluation of trace metal concentrations in some herbs and herbal teas by principal component analysis , 2009 .

[21]  J. Luten,et al.  Identification of fish species after cooking by SDS-PAGE and urea IEF: a collaborative study. , 2000, Journal of agricultural and food chemistry.

[22]  R. Stephan,et al.  Conventional and real-time PCR-based approaches for molecular detection and quantitation of bovine species material in edible gelatin. , 2005, Journal of food protection.

[23]  Reinhard Schrieber,et al.  Gelatine Handbook: Theory and Industrial Practice , 2007 .

[24]  Guifeng Zhang,et al.  Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin , 2009 .

[25]  J. Gómez-Estaca,et al.  Physico-chemical and film-forming properties of bovine-hide and tuna-skin gelatin: A comparative study , 2009 .

[26]  Rajeev Bhat,et al.  Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins , 2009 .

[27]  K. Draget,et al.  Mechanical properties of mammalian and fish gelatins based on their weight average molecular weight and molecular weight distribution. , 2009 .

[28]  M. Yılmaz,et al.  A novel method to differentiate bovine and porcine gelatins in food products: nanoUPLC-ESI-Q-TOF-MS(E) based data independent acquisition technique to detect marker peptides in gelatin. , 2013, Food chemistry.

[29]  S. Hidaka,et al.  Effects of gelatins on calcium phosphate precipitation: a possible application for distinguishing bovine bone gelatin from porcine skin gelatin , 2003 .

[30]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[31]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[32]  Lin Peng,et al.  Fingerprint profile of active components for Artemisia selengensis Turcz by HPLC–PAD combined with chemometrics , 2011 .

[33]  Guoying Li,et al.  Physicochemical properties of collagen, gelatin and collagen hydrolysate derived from bovine limed split wastes , 2006 .

[34]  M. Montowska,et al.  SPECIES IDENTIFICATION OF MEAT BY ELECTROPHORETIC METHODS , 2007 .

[35]  H. Steinhart,et al.  Characterization, antigenicity and detection of fish gelatine and isinglass used as processing aids in wines , 2010, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.